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PC226L Anti-Oxytocin Rabbit pAb

Anti-Oxytocin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC226L
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes oxytocin.
Catalogue NumberPC226L
Brand Family Calbiochem®
Application Data
Detection of rat oxytocin by immunohistochemistry. Sample: Rat hypothalamus. Primary antibody: Anti-Oxytocin Rabbit pAb (Cat. No. PC226L) (1:400). Detection: fluorescence.
References
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat supraoptic, hypothalamus, or paraventricular
Preservative≤0.1% sodium azide
Applications
Application ReferencesFree Floating Sections Maruyama, M., et al. 1999. Endocrinology 140, 2326.
Key Applications Free-floating Sections
Frozen Sections
Immunofluorescence
Application NotesFree Floating Sections (see application references)
Frozen Sections (1:4000-1:8000, ABC detection; 1:500-1:1000, PAP detection)
Immunofluorescence (1:200-1:400, indirect)
Application CommentsAntibody specificity was examined in the rat supraoptic/hypothalamus and paraventricular/hypothalamus. Staining is completely eliminated by pretreatment of antibody with oxytocin (5 µg/ml). Preabsorption of antibody with vasopressin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrat oxytocin
ImmunogenRat
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. During shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC226L 0

Documentation

Anti-Oxytocin Rabbit pAb Certificates of Analysis

TitleLot Number
PC226L

References

Reference overview
Sudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.

Citations

Title
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision09-July_2007 RFH
    ApplicationFree Floating Sections (see application references)
    Frozen Sections (1:4000-1:8000, ABC detection; 1:500-1:1000, PAP detection)
    Immunofluorescence (1:200-1:400, indirect)
    Application Data
    Detection of rat oxytocin by immunohistochemistry. Sample: Rat hypothalamus. Primary antibody: Anti-Oxytocin Rabbit pAb (Cat. No. PC226L) (1:400). Detection: fluorescence.
    DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the oxytocin protein.
    BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. Oxytocin, a neurohypophyseal hormone, has a wide range of behavioral effects in addition to classic peripheral endocrine functions. Oxytocin involvement in the central nervous system has a demonstrated role in learning and memory. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
    HostRabbit
    Immunogen speciesRat
    Immunogenrat oxytocin
    IsotypeIgG
    Speciesrat
    Positive controlRat supraoptic, hypothalamus, or paraventricular
    FormLyophilized
    FormulationUndiluted serum.
    Preservative≤0.1% sodium azide
    CommentsAntibody specificity was examined in the rat supraoptic/hypothalamus and paraventricular/hypothalamus. Staining is completely eliminated by pretreatment of antibody with oxytocin (5 µg/ml). Preabsorption of antibody with vasopressin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. During shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
    Toxicity Highly Toxic
    ReferencesSudhof, T.C. 1995. Nature 375, 645.
    Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
    Citation
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    		•
    Application referencesFree Floating Sections Maruyama, M., et al. 1999. Endocrinology 140, 2326.