Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC362 Anti-Orexin A (14-33) (Ab-2) Rabbit pAb

View Products on Sigmaaldrich.com
PC362
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.

Catalogue NumberPC362
Brand Family Calbiochem®
Application Data
Detection of human Orexin A by staining free-floating sections. Sample: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-2) Rabbit pAb (Cat. No. PC362) (1:3500).
References
ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.
Product Information
FormLiquid
FormulationSerum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
Positive controlMouse or rat brain
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Free-floating Sections
Frozen Sections
Immunofluorescence
Application NotesELISA (see comments)
Free-floating Sections (1:3500)
Frozen Sections (1:3500)
Immunofluorescence (1:2000)
Application CommentsImmunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
PC362 0