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OP181 Anti-ΔNp73 Mouse mAb (38C674)

This Anti-ΔNp73 Mouse mAb (38C674) is validated for use in Immunoblotting for the detection of ΔNp73.

Anti-ΔNp73 Mouse mAb (38C674) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP181
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP181-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~73 kDa ΔNp73 protein.
Catalogue NumberOP181
Brand Family Calbiochem®
Application Data
Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
Yang, A., et al. 2000. Nature 404, 99.
Ueda, Y., et al. 1999. Oncogene 18, 4993.
Kaghad, M., et al. 1997. Cell 90, 809.
Product Information
FormLiquid
FormulationIn PBS, 0.2% gelatin.
Positive controlCell lines transfected with ΔNp73
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
Application CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
ImmunogenHuman
Clone38C674
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP181-100UG 04055977208856

Documentation

Anti-ΔNp73 Mouse mAb (38C674) SDS

Title

Safety Data Sheet (SDS) 

Anti-ΔNp73 Mouse mAb (38C674) Certificates of Analysis

TitleLot Number
OP181

References

Reference overview
Levrero, M., et al. 2000 J. Cell Sci. 113, 1661.
Yang, A., et al. 2000. Nature 404, 99.
Ueda, Y., et al. 1999. Oncogene 18, 4993.
Kaghad, M., et al. 1997. Cell 90, 809.

Citations

Title
  • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
    Application Data
    Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody. Recognizes the ~73 kDa p73 protein lacking the N-terminal transactivation domain, ΔpNp73.
    Backgroundp73 is a relative of tumor suppressor protein p53. It displays a high homology with p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 suggests that they may have overlapping functions involved in the regulation of gene expression. The disruption of p73 leads to hippocampal dysgenesis, hydrocephalus, chronic inflammation, and infections. Splicing variant mRNAs of p73 have been identified in MCF7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxyl-terminal structures, suggesting that this region of p73 may be important for the functions of this protein. Researchers have recently cloned a p73 variant that lacks an amino-terminal transactivation domain, called ΔNp73. The ΔNp73 message is transcribed from an alternative promoter located in intron 3.
    HostMouse
    Immunogen speciesHuman
    Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
    Clone38C674
    IsotypeIgG₁
    Specieshuman
    Positive controlCell lines transfected with ΔNp73
    FormLiquid
    FormulationIn PBS, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
    Yang, A., et al. 2000. Nature 404, 99.
    Ueda, Y., et al. 1999. Oncogene 18, 4993.
    Kaghad, M., et al. 1997. Cell 90, 809.
    Citation
  • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.
    		•