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ABN78 Anti-NeuN Antibody (rabbit)

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ABN78
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HICC, IHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN78
DescriptionAnti-NeuN Antibody (rabbit)
Alternate Names
  • RNA binding protein fox-1 homolog 3
  • Fox-1 homolog C
  • Fox-3
  • Hexaribonucleotide-binding protein 3
  • NeuN
  • Neuronal nuclei
Background InformationRNA binding protein fox-1 homolog 3 (UniProt Q8BIF2; also known as Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.
Product Information
FormatAffinity Purified
Control
  • Mouse brain nuclear extract
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NeuN (rabbit polyclonal), Cat. No. ABN78, is rabbit polyclonal antibody that detect NeuN and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected NeuN in Mouse Brain Nuclear Extract.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NeuN in human cerebral cortex and mouse hippocampus tissue sections.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in E18 neuronal cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine NeuN.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects NeuN. It targets an epitope within first 97 amino acids from the N-terminal region.
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Snail based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rbfox3
  • D11Bwg0517e
  • Hrnbp3
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~48/42 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse E16 Brain lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NeuN in Mouse E16 Brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
ABN78 04053252292583