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AB3212-I Anti-Na+/HCO3- Cotransporter Antibody

Detect Na+/HCO3- Cotransporter using this Anti-Na+/HCO3- Cotransporter Antibody validated for use in Western Blotting, Immunohistochemistry.

Anti-Na+/HCO3- Cotransporter Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Uni Prot Number: Q9Y6R1
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AB3212-I
100 μg  
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Key Spec Table

Uni Prot Number
Q9Y6R1
Description
Catalogue NumberAB3212-I
DescriptionAnti-Na+/HCO3- Cotransporter Antibody
Alternate Names
  • Electrogenic sodium bicarbonate cotransporter 1
  • Sodium bicarbonate cotransporter
  • Na(+)/HCO3(-) cotransporter
  • Solute carrier family 4 member 4
  • kNBC1
Background InformationElectrogenic sodium bicarbonate cotransporter 1 (UniProt Q9Y6R1; also known as Sodium bicarbonate cotransporter, Na(+)/HCO3(-) co-transporter, Solute carrier family 4 member 4, kNBC1) is encoded by the SLC4A4 (also known as NBC, NBC1, NBCE1) gene (Gene ID 8671) in human. Bicarbonate (HCO3-) transporters are the principal regulators of pH in cells and play a vital role in acid-base movement in various tissues. There exist four types of HCO3- transporters, namely Cl-/-HCO3- exchangers, Na+/HCO3- co-transporters (NBCs), a K+/HCO3- co-transporter, and a Na+-driven Cl-/-HCO3- exchanger. SLC4A4 is a multi-pass membrane NBC with 10 transmembrane segments, 5 extracellular domains, and 6 intracellular domains, having both its N- and C-terminal (a.a. 1-468 and a.a. 987-1079, respectively) exposed intracellularly. NBCs mediate the coupled movement of sodium and bicarbonate ions, in a 3 to 1 stoichiometric ratio, across the plasma membrane. Multiple isoforms are reported as a result of alternative splicing, including NBCe1-A (kNBC1, kNBC-1; UniProt Q9JI66-2/Rat and Q9Y6R1-2/Human) that is mainly expressed in the kidney, NBCe1-B (bNBC1, pNBC-1; UniProt Q9JI66-1/Rat and Q9Y6R1-1/Human) expressed in the pancreas and other tissues, and NBCe1-C (b2NBC; UniProt Q9JI66-3/Rat and Q9Y6R1-5/Human) expressed in the brain.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Na+/HCO3- Cotransporter using this Anti-Na+/HCO3- Cotransporter Antibody validated for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Na+/HCO3- cotransporter in human cerebral cortex, kidney and pancreas tissues.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to amino acids 338-391 of human Na+/HCO3- cotransporter.
EpitopeAmino acids 338-391
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all rat and human spliced isoforms reported by UniProt (Q9JI66 and Q9Y6R1, respectively).
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Rabbit based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC4A4
  • NBC
  • NBC1
  • NBCE1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Na+/HCO3- cotransporter in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3212-I 04055977315707