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05-432 Anti-NR1 Antibody, CT

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05-432
200 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-432
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-NR1 Antibody, CT
Alternate Names
  • Grin1
  • NMDA R1 receptor C1 cassette
  • glutamate receptor, ionotropic
  • N-methyl D-aspartate 1
Background InformationNR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Product Information
FormatPurified
Control
  • Brain tissue
PresentationPurified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation:
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.

Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.

Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
Biological Information
ImmunogenHis-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.
EpitopeThe epitope maps to a region (a.a. 834-864) common to all NR1 isoforms
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteRat. Immunoreactivity is predicted for human and mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NR1
  • Grin1
  • Nmdar1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein, Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities.

POST-TRANSLATIONAL MODIFICATION: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

SIZE: 938 amino acids; 105,509 Da
Molecular Weight130 kDa observed
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on rat brain microsomal protein preparation.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2° to 8ºC from date of receipt.
Packaging Information
Material Size200 µg
Global Trade Item Number
Catalogue Number GTIN
05-432 04053252674723