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MABN2301-100UG Anti-NLGN4X Antibody, clone CSTEM30

Anti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.

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MABN2301-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABN2301-100UG
DescriptionAnti-NLGN4X Antibody, clone CSTEM30
Alternate Names
  • Neuroligin-4 X-linked
  • Neuroligin X
  • HNLX
Background InformationNeuroligin-4, X-linked (UniProt: Q8N0W4; also known as Neuroligin X, HNLX) is encoded by the NLGN4X (also known as KIAA1260, NLGN4, UNQ365/PRO701) gene (Gene ID: 57502) in human. NLGN4X is a single-pass type I membrane, homodimeric, glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 42-676) a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). It is predominantly expressed in the heart tissue. It is also expressed at lower levels in the liver, skeletal muscle, pancreas, and brain. It is a putative neuronal cell surface protein that is involved in cell-cell-interactions. In human, NLGN4X is localized at excitatory synapses, whereas in mouse NLGN4-like is localized at glycinergic synapses. NLGN4X can be phosphorylated by protein kinase C that enhances excitatory synapses. Mutations in NLGN4X gene have been linked to X-linked Asperger syndrome and Autism. (Ref.: Nguyen, TA., et al. (2020). Front. Synaptic Neurosci. 12; 33; Laumonnier, F., et al. (2004). Am. J. Hum. Genet. 74(3); 552-557).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody Ig G2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • ELISA
Application NotesTested applications

Flow Cytometry Analysis: A representative lot detected NLGN4X in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640; Liu, X., et al. (2017). Nat Methods. 14(11):1055-1062; Liu, X., et al. (2020). Nature. 586(7827):101-107).

Immunocytochemistry Analysis: A representative lot detected NLGN4X in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

ELISA Analysis: A representative lot detected NLGN4X in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant protein fragment corresponding to the extracellular domain of human Neuroligin-4,x-linked (NLGN4X).
EpitopeExtracellular domain
CloneCSTEM30
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CSTEM30 is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X). It targets an epitope within the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NLGN4X
Purification MethodProtein G purified
UniProt Number
Molecular Weight91.92 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HFF-hiPSCs.

Flow Cytometry Analysis (FC): 1 µg of this antibody detected NLGN4X in one million Human foreskin fibroblast-induced pluripotent stem cells (HFF-hiPSCs) .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2301-100UG 04065268900673