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AB16528 Anti-NIK Antibody, CT

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AB16528
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB16528
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-NIK Antibody, CT
Alternate Names
  • NFkB inducing Kinase
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NIK Antibody, C-terminus is an antibody against NIK for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot

293 cell lysate can be used as positive control.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenRabbit anti-NIK polyclonal antibody was raised against a peptide corresponding to amino acids 931 to 947 of human NIK (Malinin et al. 1997).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, LPS and mitogens. A serine/threonine protein kinase which mediates NF-kB activation by IL-1, TNFa and CD95 was identified recently and designated NIK (for NF-kB inducing kinase) (Malinin et al. 1997). NIK is an activator of IkB kinase alpha and beta (IKKalpha and IKKbeta) (Regnier et al. 1997; Woronicz et al. 1997; Ling et al. 1998; Nakano et al. 1998). Therefore, NIK is a key molecule in the NF-kB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
Gene Symbol
  • MAP3K14
  • hsNIK
  • FTDCR1B
  • HSNIK
  • HS
  • NIK
  • HsNIK
  • EC 2.7.11.25
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q99558 # Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Induces the processing of NF-kappa-B 2/P100. Could act in a receptor-selective manner (By similarity).
SIZE: 947 amino acids; 104042 Da
SUBUNIT: Binds to TRAF2, TRAF5, TRAF6, IKKA and NF-kappa-B 2/P100 (By similarity). Interacts with PELI3.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
PTM: Autophosphorylated.
SIMILARITY: SwissProt: Q99558 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB16528 04053252333828

Documentation

Anti-NIK Antibody, CT SDS

Title

Safety Data Sheet (SDS) 

Anti-NIK Antibody, CT Certificates of Analysis

TitleLot Number
RABBIT ANTI-NIK (C-TERMINUS) POLYCLONAL ANTIBODY - 2424465 2424465
RABBIT ANTI-NIK (C-TERMINUS) POLYCLONAL ANTIBODY 2903075
RABBIT ANTI-NIK (C-TERMINUS) POLYCLONAL ANTIBODY 2979900
RABBIT ANTI-NIK (C-TERMINUS) POLYCLONAL ANTIBODY - 2363538 2363538

References

Reference overviewPub Med ID
NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176.
Ling, L, et al.
Proc. Natl. Acad. Sci. U.S.A., 95: 3792-7 (1998)  1998

Show Abstract
9520446 9520446
IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK.
Woronicz, J D, et al.
Science, 278: 866-9 (1997)  1997

Show Abstract
9346485 9346485
MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.
Malinin, N L, et al.
Nature, 385: 540-4 (1997)  1997

Show Abstract
9020361 9020361

Brochure

Title
Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling