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MAB1567 Anti-Myelin Associated Glycoprotein Antibody, clone 513

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MAB1567
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, H, M, R, FgICC, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1567
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Myelin Associated Glycoprotein Antibody, clone 513
Alternate Names
  • MAG
Background InformationMyelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.
Product Information
FormatPurified
Control
  • Rat hippocampus tissue, rat brain lysate, PC12 cells.
PresentationPurified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationDetect Myelin Associated Glycoprotein using this Anti-Myelin Associated Glycoprotein Antibody, clone 513 validated for use in IC, IH & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blot:
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}

Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenL2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.
Clone513
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityDetects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Rat
  • Frog
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
Gene Symbol
  • MAG
  • SIGLEC4A
  • SIGLEC-4A
  • Siglec-4a
  • S-MAG
  • GMA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P20916 # Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3- linked sialic acid (By similarity).
SIZE: 626 amino acids; 69069 Da
SUBUNIT: Binds to RTN4R (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P20916 ## Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. & Contains 4 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight100 kDa
Product Usage Statements
Quality AssuranceEvaluated by immunohistochemistry on rat hippocampus.

Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB1567 04053252359828