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MABC1608-25UG Anti-Mucin-16 (CA-125) Antibody, clone 5E11

Detect Mucin-16 using this mouse monoclonal Anti-Mucin-16 (CA-125), clone 5E11 Antibody, Cat. No. MABC1608-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting, Proximity Ligation Assay.

Anti-Mucin-16 (CA-125) Antibody, clone 5E11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MUC16, CA125 UniProt Number: Q8WXI7
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MABC1608-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WB, Proximity Ligation AssayMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1608-25UG
DescriptionAnti-Mucin-16 (CA-125) Antibody, clone 5E11
Alternate Names
  • MUC-16
  • Ovarian cancer-related tumor marker CA125
  • CA-125
  • Ovarian carcinoma antigen CA125
Background InformationMucin-16 (UniProt: Q8WXI7; also known as MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125) is encoded by the MUC16 (also known as CA125) gene (Gene ID: 94025) in human. Mucin-16 is a heavily O-glycosylated protein that can be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus, which induces proteolytic cleavage and liberation of extracellular domain. I provides protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Mucin-16 is composed of three domains: a Serine, Threonine-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane domain with a short cytoplasmic tail. Its expression is up-regulated in ovarian cancer cells and may serve as a marker for ovarian cancer. Mucin-16 is shown to binds to mesothelin (MSLN) and this binding mediates heterotypic cell adhesion, which may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Mucin-16 using this mouse monoclonal Anti-Mucin-16 (CA-125), clone 5E11 Antibody, Cat. No. MABC1608-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting, Proximity Ligation Assay.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • Proximity Ligation Assay
Application NotesImmunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).
Proximity Ligation Assay Analysis: A representative lot detected Mucin-16 in Proximity Ligation Assay applications (Ricardo, S., et. al. (2016). Virchows Arch. 468(6):715-22; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).
Immunohistochemistry Analysis: A representative lot detected Mucin-16 in Immunohistochemistry applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).
Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).
Western Blotting Analysis: A representative lot detected Mucin-16 in Western Blotting applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 264 amino acids from the C-terminal half of human Mucin-16.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5E11 detects Mucin-16 in human ovarian cancer tissue. It targets an epitope within 264 amino acids from the C-terminal half.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MUC16
  • CA125
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human ovarian cancer and human uterine tissues.

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution of this antibody detected Mucin-16 in human ovarian cancer and human uterine tissues.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1608-25UG 04054839437885