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ABC2044-100UG Anti-Muc2-D3

Anti-Muc2-D3, Cat. No. ABC2044, is a rabbit polyclonal antibody that detects Human Mucin-2 protein and is teste for use in Electron Microscopy and Western Blotting.

ABC2044-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABC2044-100UG
DescriptionAnti-Muc2-D3
Alternate Names
  • Mucin-2 D3 domain
Background InformationMucin-2 (UniProt: Q02817; also known as MUC-2, Intestinal Mucin-2) is encoded by the MUC2 (also known as SMUC) gene (Gene ID: 4583) in human. Mucin-2 (MUC2) is a secreted protein that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Mucin-2 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. It exists as a disulfide-linked homotrimer that can be highly O-glycosylated. It contains four VWFD domains and two VWFC domains. Mucin-2 undergoes proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer that allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. The numerous O-glycans on the Mucin-2 are reported to serve as nutrients for the bacteria, serve as attachment sites, and probably contribute to the selection of the species-specific colon flora. Reduced levels of Mucin-2 have been linked to the early stage of colorectal cancer development, which is caused by methylation of the MUC2 promoter. This polyclonal antibody detects D3 domain of mucin-2, which is localized in amino acids 858-1259. (Ref.: Wi, D-H., et al. (2021). BMB Rep. 54(7); 344-355; Javitt, G., et al. (2020). Cell. 183(3); 717-729; Javitt, G., et al. (2019). J. Mol. Biol. 431(19); 3740-3752; Johansson, MEV., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(Suppl. 1); 4659-4665).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Muc2-D3, Cat. No. ABC2044, is a rabbit polyclonal antibody that detects Human Mucin-2 protein and is teste for use in Electron Microscopy and Western Blotting.
Key Applications
  • Western Blotting
  • Electron Microscopy
Application NotesTested Applications

Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).

Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.
EpitopeN-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects D3 domain of Mucin-2. It targets an epitope within D3 domain from the N-terminal half.
IsotypeIgGκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MUC2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed for D3 domain; 550.85 kDa calculated for Mucin-2. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with Human Muc2-D3 protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC2044-100UG 04065270453563