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444105 Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)

This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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444105
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, MkMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
444105-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~300 kDa mannose 6-phosphate receptor protein.
Catalogue Number444105
Brand Family Calbiochem®
References
ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml, non-reducing, see comments)
Immunofluorescence (see comments)
Immunohistochemistry (2-5 µg/ml)
Immunoprecipitation (see comments)
Application CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
ImmunogenBovine
Epitopewithin the extracelluar domain
Clone2G11
HostMouse
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444105-100UG 04055977205411

Documentation

Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) SDS

Title

Safety Data Sheet (SDS) 

Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) Certificates of Analysis

TitleLot Number
444105

References

Reference overview
Dintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
ApplicationImmunoblotting (1 µg/ml, non-reducing, see comments)
Immunofluorescence (see comments)
Immunohistochemistry (2-5 µg/ml)
Immunoprecipitation (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
BackgroundLysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of ~300 kDa cation-independent MPRs. The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPRs is predominantly late endosomes, fewer in the trans Golgi network, and ~10% at cell surface. In addition to its man6P binding activity, MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form of the extracellular domain of MPR has been detected circulating in serum that is capable of binding both ligands.
HostMouse
Immunogen speciesBovine
Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
Epitopewithin the extracelluar domain
Clone2G11
IsotypeIgG2a
Speciesbovine, not hamster, human, monkey
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.