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MABT846-25UG Anti-MYH-1 Antibody, clone 6F12H3

Anti-MYH-1, clone 6F12H3, Cat. No. MABT846, is a rat monoclonal antibody that detects Myosin-1 and has been tested for use in Immunofluorescence. Immunohistochemistry, and Western Blotting.

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MABT846-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABT846-25UG
DescriptionAnti-MYH-1 Antibody, clone 6F12H3
Alternate Names
  • Myosin-1
  • Myosin heavy chain 1
  • Myosin heavy chain 2x
  • MyHC-2x
  • Myosin heavy chain skeletal muscle adult 1
Background InformationMyosin-1 (UniProt: Q5SX40; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain, skeletal muscle, adult 1) is encoded by the Myh1 gene (Gene ID: 17879) in murine species. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-MYH-1, clone 6F12H3, Cat. No. MABT846, is a rat monoclonal antibody that detects Myosin-1 and has been tested for use in Immunofluorescence. Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-1 in mouse heart and mouse skeletal muscle tissue.

Immunofluorescence Analysis: A representative lot detected MYH-1 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Western Blotting Analysis: A representative lot detected MYH-1 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunohistochemistry Analysis: A representative lot detected MYH-1 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-1.
Clone6F12H3
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 6F12H3 is a rat monoclonal antibody that detects Myosin-1 in mouse and rat muscles. It target an epitope within the coiled coil domain in the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Myh1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~220 kDa observed; 223.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-1 in mouse soleus muscle tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT846-25UG 04054839495946

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