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MABF2202-25UG Anti-MOSPD2 Antibody, clone 1MOS‐4E10

Anti-MOSPD2, clone 1MOS‐4E10, Cat. No. MABF2202, is a mouse monoclonal antibody that detects Motile sperm domain-containing protein 2 and is tested for use in Immunohistochemistry and Western Blotting.

MABF2202-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2202-25UG
DescriptionAnti-MOSPD2 Antibody, clone 1MOS‐4E10
Alternate Names
  • Motile sperm domain-containing protein 2
Background InformationMotile sperm domain-containing protein 2 (UniProt: Q8NHP6; also known as MOSPD2 is encoded by the MOSPD2 gene (Gene ID: 158747) in human. MOSPD2 is a single-pass FFAT-motif (two phenylalanines (FF) in an acidic track (AT)) interacting membrane protein. It binds to FFAT motif via its major sperm protein (MSP) domain (aa 327-445). MOSPD2 is an endoplasmic reticulum (ER)-anchored protein that interacts with several FFAT-containing tether proteins from endosomes, mitochondria, and Golgi and mediate the formation of contact sites between the ER and these organelles. MOSPD2 is highly expressed in CD14+ monocytes and at lower levels in neutrophils. Its expression is not observed in B- and T- cells. It is reported to promote migration of primary monocytes and neutrophils, in response to various chemokines. It consists of three structural domains: an N-terminal CRAL/TRIO domain that is involved in small lipophilic molecule binding, a MSP domain, and a carboxyl terminal transmembrane domain. Two isoforms of MOSPD2 have been described that are produced by alternative splicing. (Ref.: Di Mattia, T., et al. (2018). EMBO Rep.19(7): e45453).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-MOSPD2, clone 1MOS‐4E10, Cat. No. MABF2202, is a mouse monoclonal antibody that detects Motile sperm domain-containing protein 2 and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected MOSPD2 in Panc-1 cell lysate.

Western Blotting Analysis: A representative lot detected MOSPD2 in Western Blotting applications (Di Mattia, T., et. al. (2018). EMBO Rep. 19(7); e45453).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenA recombinant fragment corresponding to 119 amino acid MSP domain of human Motile sperm domain-containing protein 2 (MOSPD2).
Clone1MOS‐4E10
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1MOS‐4E10 is a mouse monoclonal antibody that detects human MOSPD2. It targets an epitope within the MSP domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MOSPD2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~56 kDa observed; 59.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human pancreas tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected MOSPD2 in human pancreas tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2202-25UG 04061841844501

Documentation

Anti-MOSPD2 Antibody, clone 1MOS‐4E10 SDS

Title

Safety Data Sheet (SDS) 

Anti-MOSPD2 Antibody, clone 1MOS‐4E10 Certificates of Analysis

TitleLot Number
Anti-MOSPD2, clone 1MOS‐4E10 - Q3437414 Q3437414

References

Reference overviewPub Med ID
Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites.
Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nominé Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F.
EMBO Rep  19(7)  e45453  2018

Show Abstract 		29858488 29858488