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PC342 Anti-MMP-2 (Ab-7) Rabbit pAb

Anti-MMP-2 (Ab-7) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2

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PC342
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~72 kDa latent form of the MMP-2 protein. Cross-reacts with recombinant MMP-9 at high concentrations but does not cross-react with native MMP-9.
Catalogue NumberPC342
Brand Family Calbiochem®
SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
References
ReferencesNikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
Product Information
FormLiquid
FormulationIn 1X PBS, 0.2% BSA.
Positive controlConditioned medium from HT-1080 cells or recombinant MMP-2
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Frozen Sections
Application NotesFrozen Sections (not recommended
Immunoblotting (2 µg/ml)
Immunoprecipitation (1 µg/sample)
Application CommentsThis antibody will cross-react with recombinant human MMP-9 at high concentrations of protein. No cross-reactivity seen with native MMP-9. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human pro-MMP-2
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC342 0

Documentation

Anti-MMP-2 (Ab-7) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-MMP-2 (Ab-7) Rabbit pAb Certificates of Analysis

TitleLot Number
PC342

References

Reference overview
Nikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
ApplicationFrozen Sections (not recommended
Immunoblotting (2 µg/ml)
Immunoprecipitation (1 µg/sample)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~72 kDa latent form of MMP-2.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase, type IV collagenase, TBE-1) is synthesized as a 631 amino acid proenzyme (72 kDa zymogen) which is proteolytically processed at the first 80 amino acids to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that MMP-2 may be a useful marker for the diagnosis or prognosis of cancer.
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant, human pro-MMP-2
IsotypeIgG
Specieshuman, mouse
Positive controlConditioned medium from HT-1080 cells or recombinant MMP-2
FormLiquid
FormulationIn 1X PBS, 0.2% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody will cross-react with recombinant human MMP-9 at high concentrations of protein. No cross-reactivity seen with native MMP-9. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesNikkari, S.T., et al. 1996. Am. J. Pathol. 149, 1427.
Soini, Y., et al. 1994. J. Histochem. And Cytochemistry 42, 945.
Autio-Harmainen, H., et al. 1993. Lab. Invest. 69, 312.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Schnaper, H.W., et al. 1993. J. Cell. Physiol. 156, 235.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Autio-Harmainen, H., et al. 1992. Lab. Invest. 67, 191.
Fridman, R., et al. 1992. J. Biol. Chem. 267, 15398.
Margulies, I.M., et al. 1992. Cancer Epidemiology, Biomarkers and Prevention 1, 467.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.