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IM64 Anti-MMP-13 (Ab-3) Mouse mAb (181-14G11)

Anti-MMP-13 (Ab-3) Mouse mAb (181-14G11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Collagenase-3, Anti-Matrix Metalloproteinase 13

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Overview

Replacement Information

Key Spec Table

Host
M
Description
OverviewRecognizes the ~54-60 kDa latent and the ~48 kDa active forms of MMP-13.
Catalogue NumberIM64
Brand Family Calbiochem®
SynonymsAnti-Collagenase-3, Anti-Matrix Metalloproteinase 13
References
ReferencesShlopov, B., et al. 1997. Arthritis Rheum. 40, 2065. Knauper V., et al. 1996. J. Biol. Chem. 271, 1544. Freije, J.M.P., et al. 1994. J. Biol. Chem. 269, 16766. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Positive controlConditioned medium from PMA-treated HT-1080 cells or breast, bladder, or ovarian carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml) Paraffin Sections (2.5 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human pro-MMP-13
ImmunogenHuman
Clone181-14G11
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM64 0

Documentation

References

Reference overview
Shlopov, B., et al. 1997. Arthritis Rheum. 40, 2065. Knauper V., et al. 1996. J. Biol. Chem. 271, 1544. Freije, J.M.P., et al. 1994. J. Biol. Chem. 269, 16766. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-February-2008 JSW
SynonymsAnti-Collagenase-3, Anti-Matrix Metalloproteinase 13
ApplicationImmunoblotting (1 µg/ml) Paraffin Sections (2.5 µg/ml)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~54-60 kDa latent and the ~48 kDa active forms of MMP-13.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-13 (MMP-13) also known as collagenase-3 is secreted as a 60 kDa proenzyme which is proteolytically processed to the 48 kDa active MMP-13 form. MMP-13 shows substrate specificity toward interstitial collagens I-III and gelatin, preferentially cleaving type II collagen over types I and III and cleaving fibrillar type I collagen with comparable efficiency to MMP-1 and MMP-8. MMP-13 is expressed in breast carcinomas and may be important in the turnover of articular cartilage, which is rich in type II collagen. Active MMP-13 can be inhibited in vitro by chelators of divalent ions such as EDTA and o-phenanthroline and in vivo by TIMP-1, TIMP-2, and TIMP-3. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that it may be a useful marker for the diagnosis or prognosis of cancer.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant human pro-MMP-13
Clone181-14G11
IsotypeIgG₁
Specieshuman
Positive controlConditioned medium from PMA-treated HT-1080 cells or breast, bladder, or ovarian carcinoma tissue
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesShlopov, B., et al. 1997. Arthritis Rheum. 40, 2065. Knauper V., et al. 1996. J. Biol. Chem. 271, 1544. Freije, J.M.P., et al. 1994. J. Biol. Chem. 269, 16766. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.