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MAB16200-AF488 Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate

Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immuno

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Research Category: Epigenetics & Nuclear Function Gene Symbol: MGMT UniProt Number: P16455
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MAB16200-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB16200-AF488
DescriptionAnti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate
Alternate Names
  • Methylated-DNA-protein-cysteine methyltransferase
  • 6-O-methylguanine-DNA methyltransferase
  • GATase
  • MGMT
  • O-6-methylguanine-DNA-alkyltransferase
Background InformationMethylated-DNA-protein-cysteine methyltransferase (EC 2.1.1.63; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.
Product Information
FormatAlexaFluor®488
PresentationPurified mouse IgG1 Alexa Fluor® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesFlow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.

This monoclonal antibody is also available in unconjugated and Alexa Fluor® 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
Biological Information
ImmunogenGATase purified from CEM-CCRF human leukemic lymphoblasts (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032; Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
CloneMT3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MGMT
Purification MethodProtein A purified.
UniProt Number
Molecular Weight21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor® 488-conjugated antibody detected MGMT in MCF-7 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MAB16200-AF488 04054839073489