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DR1080 Anti-MCM2 Mouse mAb (6A8)

This Anti-MCM2 Mouse mAb (6A8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of MCM2.

Anti-MCM2 Mouse mAb (6A8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Minichromosome Maintenance Complex Component 2

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~140-145 kDa MCM2 protein in HeLa cells.
Catalogue NumberDR1080
Brand Family Calbiochem®
SynonymsAnti-Minichromosome Maintenance Complex Component 2
Application Data
Detection of human MCM2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MCM2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 805-904 human MCM2, expressed as a GST fusion protein
Clone6A8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1080 0

Documentation

Anti-MCM2 Mouse mAb (6A8) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-Minichromosome Maintenance Complex Component 2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human MCM2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MCM2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~140-145 kDa MCM2 protein.
BackgroundThe protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 805-904 human MCM2, expressed as a GST fusion protein
Clone6A8
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review