Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

434629 Anti-Lewis b Mouse mAb (T218)

This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.

Anti-Lewis b Mouse mAb (T218) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Leb

View Products on Sigmaaldrich.com
434629
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
Catalogue Number434629
Brand Family Calbiochem®
SynonymsAnti-Leb
References
ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Product Information
FormLiquid
FormulationIn PBS, 1% BSA.
Positive controlColon adenocarcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (see comments)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
Application CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
Biological Information
Immunogenhuman SK-CO-10 colon cancer cells
ImmunogenHuman
CloneT218
HostMouse
IsotypeIgM
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
434629 0

Documentation

Anti-Lewis b Mouse mAb (T218) SDS

Title

Safety Data Sheet (SDS) 

Anti-Lewis b Mouse mAb (T218) Certificates of Analysis

TitleLot Number
434629

References

Reference overview
Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-November-2018 JSW
SynonymsAnti-Leb
ApplicationELISA (see comments)
Immunoblotting (see comments)
Immunohistochemistry (1:10-1:40)
DescriptionMouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
BackgroundBlood group antigens are a group of molecules formed by sequential addition of saccharides to carbohydrate side chains of lipids and proteins and are detected on erythrocytes, certain epithelial cells and in secretions of some individuals. A, B and H antigens are synthesized by erythrocytes and integrated into their cell membranes. Lewis antigens are not synthesized by erythrocytes but can be absorbed from the plasma onto the surface of red blood cells. Subtle differences in the composition and arrangement of the sugars in the chains are sufficient to generate these distinct specificities. Lewis b may be detected in gastrointestinal and colorectal tumors and pancreatic cancer.
HostMouse
Immunogen speciesHuman
Immunogenhuman SK-CO-10 colon cancer cells
CloneT218
IsotypeIgM
Specieshuman
Positive controlColon adenocarcinoma tissue
FormLiquid
FormulationIn PBS, 1% BSA.
Preservative≤0.1% sodium azide
CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.