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MAB2041-I-25UL Anti-Laminin β1 Antibody, clone 3E5

This mouse monoclonal Anti-Laminin β1, clone 3E5, Cat. No. MAB2041-I is tested for use in Western Blotting and ELISA, Inhibition, and Electron Microscopy, for the detection of Laminin subunit β1.

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MAB2041-I-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMAB2041-I-25UL
DescriptionAnti-Laminin β1 Antibody, clone 3E5
Alternate Names
  • Laminin subunit beta-1
  • Laminin B1 chain
  • Laminin-1 subunit beta
Background InformationLaminin subunit b1 (UniProt: P07942; also known as Laminin B1 chain, Laminin-1 subunit beta) is encoded by the LAMB1 gene (Gene ID: 3912) in human. Laminins are the major non-collagenous constituent of basement membranes that participate in wide variety of biological processes, including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. They are a family of heterotrimeric extracellular glycoproteins consisting of α, β, and γ chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five α, three β, and three γ subunits are described that assemble into at least 15 different laminin molecules. Different α, β, and γ chain isomers combine to give rise to different heterotrimeric laminin isoforms, which are designated by Arabic numerals in the order of their discovery. Laminin- β1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is expressed in most tissues that produce basement membranes and is one of the 3 chains constituting laminin 1. It is shown to be involved in the organization of the laminar architecture of cerebral cortex and is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the end feet of radial glial cells and as a physical barrier to migrating neurons. (Ref.: Radmanesh, F., et al. (2013). Am. J. Hum. Genet. 92(3); 468-474; Virtanen, I., et al. (1995). J. Histochem. Cytochem. 43(6); 621-628)).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in PBS without azide.
Quality LevelMQ200
Applications
ApplicationThis mouse monoclonal Anti-Laminin β1, clone 3E5, Cat. No. MAB2041-I is tested for use in Western Blotting and ELISA, Inhibition, and Electron Microscopy, for the detection of Laminin subunit β1.
Key Applications
  • Western Blotting
  • Inhibition
  • Electron Microscopy
  • ELISA
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected Laminin β1 in Western Blotting applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Electron Microscopy: A representative lot detected Laminin β1 in Electron Microscopy applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Inhibition: A representative lot inhibited the neurite-promoting activity of laminin. (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

ELISA Analysis: A representative lot detected Laminin β1 in ELISA applications (Engvall, E., et al. (1986). J Cell Biol.;103(6 Pt1):2457-65).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenLaminin fragments from human placenta.
EpitopeUnknown
Clone3E5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3E5 is a mouse monoclonal antibody that detects Laminin subunit β1. It targets an epitope near the end of long arm.
IsotypeIgG
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAMB1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 198.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human placenta tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Laminin β1 in Human placenta tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2041-I-25UL 04065266791488