Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GR07 Anti-Insulin Receptor (Ab-3) Mouse mAb (29B4)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
GR07
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~95 kDa β chain of the insulin receptor protein. Cross-reacts with IGF-I receptor.
Catalogue NumberGR07
Brand Family Calbiochem®
References
ReferencesGrigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlNormal skin fibroblasts
Positive controlHepG2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Neutralization Studies Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.
Key Applications Immunoprecipitation
Not Western Blot
Not Paraffin Sections
Neutralization Studies
Application NotesImmunoblotting (not recommended)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (not recommended)
Application CommentsThe antibody has been reported to produce partial inhibition of insulin-dependent protein-tyrosine kinase activity (see application references). Also reported to cross-react with IGF-I receptor. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpartially purified, human, placental insulin receptor
ImmunogenHuman
Clone29B4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR07 0

Documentation

Anti-Insulin Receptor (Ab-3) Mouse mAb (29B4) Certificates of Analysis

TitleLot Number
GR07

References

Reference overview
Grigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2007 RFH
ApplicationImmunoblotting (not recommended)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells (see application references). Recognizes the ~95 kDa β chain of insulin receptor.
BackgroundInsulin initiates its effects on cells through interaction with high-affinity, cell surface, glycoprotein receptors, consisting of two α (135,000 dalton) and two β (95,000 dalton) subunits linked by disulfide bonds. The α subunits bind insulin and the β subunits become phosphorylated in response to insulin. Insulin-dependent phosphorylation occurs at multiple sites near the carboxyl terminus and in the kinase domain. The receptor protein-tyrosine kinase has been implicated as the mediator of most, if not all, of the effects of insulin. Mutations that diminish both phosphorylation and internalization of the insulin receptor block insulin action, as do antibodies which inhibit its protein-tyrosine kinase activity. Cells from patients with diabetic syndromes, characterized by nonresponsiveness to insulin, exhibit reduced insulin dependent protein-tyrosine kinase activity.
HostMouse
Immunogen speciesHuman
Immunogenpartially purified, human, placental insulin receptor
Clone29B4
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlHepG2 cells
Negative controlNormal skin fibroblasts
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe antibody has been reported to produce partial inhibition of insulin-dependent protein-tyrosine kinase activity (see application references). Also reported to cross-react with IGF-I receptor. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesGrigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Application referencesOriginal Clone, Neutralization Studies Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.