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MAC138 Anti-Inntag™ 5 (IT5) Antibody, clone R19/8-11/18

Anti-Inntag 5 (IT5), clone R19/8-11/18 is a mouse monoclonal antibody that detects the Innocuous Inntag IT5 based on pollen allergen PhIp2 sequence and is tested for Immunofluorescence, Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: PHLPII UniProt Number: P43214
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MAC138
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, IF, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC138
DescriptionAnti-Inntag™ 5 (IT5) Antibody, clone R19/8-11/18
Alternate Names
  • Pollen allergen Phl p 2
  • Phl p2
Background InformationPollen allergen Phl p 2 (UniProt: P43214; also known as Allergen Phl p II, Phl p 2) is encoded by the PHLPII gene in Phleum pratense (Common timothy). Phl p2 binds to IgE and causes allergic reactions in human. Inntag 5 IT5 is a small protein domain of Phelum pratense that display strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Although the Inntag IT5 has a similar length to that of the Flag tag, it does not affect cell morphology and produces only very mild effect in the mean critical size at the G1/S transition compared to Flag tag. The structure of Inntag IT5 is based on a packed beta-sheet confirmation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Inntag 5 (IT5), clone R19/8-11/18 is a mouse monoclonal antibody that detects the Innocuous Inntag IT5 based on pollen allergen PhIp2 sequence and is tested for Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected Inntag 5 (IT5) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunoprecipitation Analysis: A representative lot detected Inntag 5 (IT5) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunofluorescence Analysis: A representative lot detected Inntag 5 (IT5) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 96 amino acids of Pollen allergen Phl p 2 chain.
CloneR19/8-11/18
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone R19/8-11/18 detects Pollen allergen Phl p 2 in lysate from HEK293 cells transfected with GFP-IT5.
IsotypeIgG2bκ
Species Reactivity
  • All
Species Reactivity NotePhaleum pratense.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PHLPII
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40.2 kDa observed; 13.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected HEK293/GFP-IT5 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 5 (IT5) in 10 µg of transfected HEK293/GFP-IT5 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAC138 04054839240584