Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

05-1530 Anti-Importin beta-1 Antibody, clone 3H14

Use Anti-Importin beta-1 Antibody, clone 3H14 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Importin beta-1 also known as Karyopherin subunit beta-1, Nuclear factor P97.

Anti-Importin beta-1 Antibody, clone 3H14 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: KPNB1, IMB1, IPO1, IPOB, Impnb, Importin-90, NTF97 UniProt Number: Q14974
View Products on Sigmaaldrich.com
05-1530
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1530
DescriptionAnti-Importin beta-1 Antibody, clone 3H14
Alternate Names
  • Karyopherin subunit beta-1
  • Nuclear factor P97
  • importin 1
  • importin 90
  • importin beta-1 subunit
  • karyopherin (importin) beta 1
  • karyopherin beta 1
Background InformationImportin beta-1 (also known as Importin subunit beta-1) facilitates nuclear protein import. This occurs either though association with a nuclear localization signal (NLS) and an adapter protein , such as an importin-alpha subunit, which which binds to nuclear, or directly where the protein acts as autonomous nuclear transport receptor.
Import of macromolecules containing a NLS requires a heterodimer of importin alpha and beta subunits also called as karyopherins. Importin alpha binds the cytoplasmic NLS-containing protein and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-Importin beta-1 Antibody, clone 3H14 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Importin beta-1 also known as Karyopherin subunit beta-1, Nuclear factor P97.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Biological Information
ImmunogenRecombinant protein corresponding to human Importin beta-1.
EpitopeUnknown
Clone3H14
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Importin beta-1.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq].
Gene Symbol
  • KPNB1
  • IMB1
  • IPO1
  • IPOB
  • Impnb
  • Importin-90
  • NTF97
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.

SUBUNIT STRUCTURE: Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones By similarity. Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota. Interacts with human respiratory syncytial virus (HRSV) protein M.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus envelope

SEQUENCE SIMILARITIES: Belongs to the importin beta family.

Contains 8 HEAT repeats.

Contains 1 importin N-terminal domain.
Molecular Weight~ 97 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Importin beta-1 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1530 04053252285066

Documentation

Anti-Importin beta-1 Antibody, clone 3H14 SDS

Title

Safety Data Sheet (SDS) 

Anti-Importin beta-1 Antibody, clone 3H14 Certificates of Analysis

TitleLot Number
Anti-Importin beta-1, clone 3H14 - 2119009 2119009
Anti-Importin beta-1, clone 3H14 - 2272143 2272143
Anti-Importin beta-1, clone 3H14 - 3221098 3221098
Anti-Importin beta-1, clone 3H14 - NRG1693921 NRG1693921
Anti-Importin beta-1, clone 3H14 -2504835 2504835
Anti-Importin beta-1, clone 3H14 -2567026 2567026
Anti-Importin beta-1, clone 3H14 -2676578 2676578
Anti-Importin beta-1, clone 3H14 -2709636 2709636
Anti-Importin beta-1, clone 3H14 -2820678 2820678