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MABF2304F-25UL Anti-IL-9 receptor Antibody, clone RZ-66 Antibody, FITC Conjugate

Anti-IL-9 receptor, clone RZ-66, FITC Conjugate, Cat. No. MABF2304F, is a rat monoclonal antibody that detects Interleukin-9 receptor and is tested for use in Flow Cytometry.

MABF2304F-25UL
25 μL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2304F-25UL
DescriptionAnti-IL-9 receptor Antibody, clone RZ-66 Antibody, FITC Conjugate
Alternate Names
  • Interleukin-9 receptor
  • IL-9R
  • CD129
Background InformationInterleukin-9 receptor (UniProt: Q01114; also known as IL-9 receptor, IL-9R, CD129) is encoded by the Il9r gene (Gene ID: 16199) in murine species. IL-9R is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-37), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 38-270), a transmembrane domain (aa 271-291), and a cytoplasmic domain (aa 291-468). It contains a WSXWS motif (aa 244-248) that is essential for proper protein folding and cell surface receptor binding. It also contains a Box-1 motif (aa 301-309) that is required for JAK interaction and/or activation. IL-9R is composed of the IL-9R a-chain and common g-chain. IL-9R is expressed on mast cells, activated T cells, ILC2 cells, and basophils. It is also selectively induced on B memory cells after primary immunization and this expression is induced by stimulation with CD40. IL-9R expression on B memory cells is shown to be essential for a normal recall antibody response in murine models. Signaling by IL-9R is shown to result in the activation of major signaling pathways involving STAT1, STAT-3, and STAT-5, MAPK, and PI3K. IL-9R signaling is shown to be pro-inflammatory and it promotes allergic inflammation and airway remodeling in lungs. It is also important in cell growth and differentiation of T cells, B cells, mast cells, hematopoietic progenitors, epithelial cells, and airway smooth muscle cells. (Ref.: Takatsuka, S., et al. (2018). Nat. Immunol. 19 (9); 1025-1034).
References
Product Information
FormatFITC
PresentationPurified rat monoclonal antibody IgG1 in buffer containing PBS 0.01 M, sodium azide, 0.1%, pH7.1 and 15 mg/mL BSA with 0.01% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL-9 receptor, clone RZ-66, FITC Conjugate, Cat. No. MABF2304F, is a rat monoclonal antibody that detects Interleukin-9 receptor and is tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 3 µg/mL from a representative lot detected IL-9 receptor in B300-19 cells.

Flow Cytometry Analysis: A representative lot detected IL-9 receptor in Flow Cytometry applications (Takatsuka, S., et. al. (2018). Nat Immunol.19(9):1025-1034).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenpCADEST1 vector carrying a gene encoding for murine IL-9R GroEL fusion protein.
EpitopeUnknown
CloneRZ-66
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone RZ-66 is a rat monoclonal antibody that detects murine IL-9 receptor.
IsotypeIgG1
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Il9r
Purification MethodProtein G purified
UniProt Number
Molecular Weight52.26 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in RAW264.7 cells.

Flow Cytometry Analysis: 2 µg of this antibody detected IL-9 receptor in one million RAW264.7 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2304F-25UL 04065265324298