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MABF2297-25UG Anti-IL-12 p70 Antibody, clone 2F7

Anti-IL-12 p70, clone 2F7, Cat. No. MABF2297, is a mouse monoclonal antibody that detects IL-12 p70 and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

MABF2297-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2297-25UG
DescriptionAnti-IL-12 p70 Antibody, clone 2F7
Alternate Names
  • Interleukin-12
  • Cytotoxic lymphocyte maturation factor
  • CLMF
  • NK cell stimulatory factor
  • NKSF
Background InformationInterleukin-12 subunit alpha/beta (UniProt: P29459/ P29460; also known as IL-12A/IL-12B, Cytotoxic lymphocyte maturation factor 35 kDa/40 kDa subunits, CLMF p35/CLMF40, IL-12 subunit p35, NK cell stimulatory factor chain 1/1, NKSF1/2) are encoded by the IL12A/IL12B (also known as NKSF1/NKSF2) genes (Gene ID: 3592/3593) in human. IL-12 is a heterodimeric pro-inflammatory cytokine composed of covalently linked p35 and p40 subunits. The two genes encoding p35 and p40 are unrelated and located on separate chromosomes. However, both genes encoding IL-12 need to be expressed coordinately in the same cells to produce the biologically active heterodimer. It has been reported that in the absence of p35 subunit p40 is secreted as a monomer or a homodimer; however, p35 subunit is secreted only when associated with p40. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naïve, resting CD4+ T cells to promote their proliferation and secretion of cytokines. Microbial products are reported to be strong inducers of IL-12 by these cells. Production of IL-12 is shown to be regulated by positive and negative regulatory mechanisms. IL-10 is considered as a potent inhibitor of IL-12 production by blocking transcription of both of its encoding genes. TGF-β is also an inhibitor of IL-12 production. It acts by reducing the stability of IL-12 p40 mRNA. IL-12 induces the production of interferon- and favors the differentiation of T helper 1 (Th1) cells and forms a link between innate resistance and adaptive immunity. (Ref.: Trinchieri, G. (2003). Nat. Rev. Immunol. 3(2); 133-146).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL-12 p70, clone 2F7, Cat. No. MABF2297, is a mouse monoclonal antibody that detects IL-12 p70 and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).
Key Applications
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-12 p70 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length recombinant human Interleukin-12A.
EpitopeUnknown
Clone2F7
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 2F7 is a mouse monoclonal antibody that detects human Interleukin-12 p70.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL12A
  • NKSF1
Purification MethodProtein G purified
UniProt Number
Molecular Weight24.87 kDa calculated and 37.17 kDa, respectively for IL-12A and IL-12B.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in THP-1 cells

Flow Cytometry Analysis: 1 µg of this antibody detected IL-12 p70 in one million THP-1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2297-25UG 04061842592159