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AP1047 Anti-IKIP1 Goat pAb

Anti-IKIP1 Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Iκ B kinase interacting protein 1 Goat pAb

AP1047
  
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Overview

Replacement Information

Key Spec Table

Host
Gt
Description
Overview

This product has been discontinued.





Recognizes the ~45 kDa IKIP1 protein in U20S cells.
Catalogue NumberAP1047
Brand Family Calbiochem®
SynonymsAnti-Iκ B kinase interacting protein 1 Goat pAb
Application Data
Lane 1: MWM Lane 2: Detection of human IKIP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from U2OS cells. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence. Lane 3: Detection of human IKIP1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for IP: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: One-half of the total amount of immunoprecipitated IKIP1. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesHofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.
Product Information
FormLiquid
FormulationIn Tris citrate/phosphate buffer, pH 7.0-8.0.
Positive controlU2OS cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (2 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human IKIP1
ImmunogenHuman
HostGoat
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1047 0

Documentation

Anti-IKIP1 Goat pAb Certificates of Analysis

TitleLot Number
AP1047

References

Reference overview
Hofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Iκ B kinase interacting protein 1 Goat pAb
ApplicationImmunoblotting (2 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application Data
Lane 1: MWM Lane 2: Detection of human IKIP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from U2OS cells. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence. Lane 3: Detection of human IKIP1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for IP: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: One-half of the total amount of immunoprecipitated IKIP1. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the 45 kDa IKIP1 protein.
BackgroundIKIP1 (I kappa B kinase interacting protein, Acc.# NP_710154) is located on chromosome 12 in close proximity to APAF1 (apoptotic protease-activating factor-1). IKIP1 was originally cloned from a yeast two hybrid screening as an IKK2 interacting protein. IKIP1 and APAF1 share a common 488 bp promoter from which the two genes are transcribed in opposite directions, presumably in a co-regulated manner. Three IKIP transcripts are generated by differential splicing and alternative exon usage and show no significant homology to other genes. IKIP1 is expressed predominantly in the lung, kidney, spleen, thymus and skeletal muscle. Like APAF1, expression of IKIP1 is enhanced by γ-irradiation, in a p53-dependent manner. When transfected into endothelial cells IKIP promotes apoptosis suggesting that it plays a role in the cell death process as well as NF-κB regulation.
HostGoat
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human IKIP1
IsotypeIgG
Specieshuman
Positive controlU2OS cells
FormLiquid
FormulationIn Tris citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.