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MAB5490-I-100UG Anti-Huntingtin Antibody, a.a. 115-129 Antibody, clone 5HU-1H6

Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6, Cat. No. MAB5490-I, is a mouse monoclonal antibody that detects Huntingtin protein and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MAB5490-I-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMAB5490-I-100UG
DescriptionAnti-Huntingtin Antibody, a.a. 115-129 Antibody, clone 5HU-1H6
Alternate Names
  • Huntington disease protein
  • HD protein
Background InformationHuntingtin (UniProt: P42858; also known as Huntington disease protein, HD protein) is encoded by the HTT (also known as HD, IT15) gene (Gene ID: 3064) in human. This protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with g-tubulin. It is expressed in the brain and is mainly found in the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. It plays a role in microtubule-mediated transport or vesicle function and shown to be necessary for neuronal survival. It is involved in synaptic vesicle trafficking, and microtubule binding. It contains a nuclear export signal (aa 2395-2404) and 5 HEAT (Huntington, Elongation Factor 3, PR65/A, TOR) domains. It can undergo cleavage by caspases downstream of the polyglutamine stretch and the resulting N-terminal fragments displays cytotoxicity and provokes apoptosis. In response to DNA damage, it undergoes phosphorylation at Serine 1179 and 1199 by CDK5 that is shown to protect neurons against polyglutamine expansion as well as DNA damage mediated toxicity. The N-terminal fragments of mutant huntingtin (mHTT) misfold and assemble into oligomers that ultimately bundle into insoluble fibrils. Misfolding and self-aggregation of specific proteins is commonly observed in well-known neurodegenerative diseases, including Huntington s disease (HD), Alzheimer s disease (AD), Parkinson s disease (PD), and amyotrophic lateral sclerosis (AML). Abnormal polyglutamine (polyQ) repeat sequence or stretch result in protein misfolding and neurodegeneration.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Huntingtin, a.a. 115-129, clone 5HU-1H6, Cat. No. MAB5490-I, is a mouse monoclonal antibody that detects Huntingtin protein and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
  • Immunocytochemistry
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected Huntingtin protein in Western Blotting application. (Miller, J.P., et al. (2010). Neuron. 67(2):199-212; Lunke, A. et al. (2002). Mol Cell. 10(2):259-69; Ratovitski, T., et al. (2007). Cell Cycle. 6(23):2970-81; Michels Thompson, L., et al. (2009). J Cell Biol. 187(7):1083-99; Juenemann, K., et al. (2011). Neurotox Res.;20(2):120-33).

Immunohistochemistry Applications: A representative lot detected Huntingtin protein in Immunohistochemistry application. (Lunke, A. et al. (2002). Mol Cell. 10(2):259-69).

Immunofluorescence Analysis: A representative lot detected Huntingtin protein in Immunofluorescence application. (Juenemann, K., et al. (2011). Neurotox Res.;20(2):120-33).

Immunocytochemistry Analysis: A representative lot detected Huntingtin protein in Immunocytochemistry application. (Miller, J.P., et al. (2010). Neuron. 67(2):199-212; Lunke, A. et al. (2002). Mol Cell. 10(2):259-69).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the first 165 amino acids from the N-terminal region of human Huntingtin protein.
EpitopeN-terminus
Clone5HU-1H6
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5HU-1H6 is a mouse monoclonal antibody that detects human Huntingtin protein. It targets an epitope within 15 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HTT
UniProt Number
Molecular Weight~350 kDa observed; 347.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from HEK293 cells transiently overexpressing Huntingtin protein.

Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Huntingtin in lysates from HEK293 cells transiently overexpressing Huntingtin protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5490-I-100UG 04065266876260

Documentation

Anti-Huntingtin Antibody, a.a. 115-129 Antibody, clone 5HU-1H6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Huntingtin Antibody, a.a. 115-129 Antibody, clone 5HU-1H6 Certificates of Analysis

TitleLot Number
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3817594 3817594
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3819906 3819906
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3823314 3823314
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3827379 3827379
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3837172 3837172
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3878882 3878882
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3884347 3884347
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3892597 3892597
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3894643 3894643
Anti-Huntingtin, a.a. 115-129, clone 5HU-1H6 - 3897355 3897355
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