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MAB1288 Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate

This Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate is validated for use in FC for the detection of HLA-B7.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: HLA-B, Bw-54, Bw-45, Bw-47, Bw-46, Bw-52, B-5, Bw-4, Bw-57, OTTHUMP00000034826, AS, Bw-56, Bw-55, Bw-41, Bw-48, B'DT, Bw-50, Bw-60, Bw-58, B-21, HLA-B-5501, B-12, Bw-22, Bw-53, HLAB, SPDA1, Bw-44 UniProt Number: P03989
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MAB1288
100 assays  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberMAB1288
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-HLA-B7 Antibody, clone BB7.1, PE Conjugate
OverviewBIOCHEMISTRY: p I = 6.3
Background InformationHLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a membrane-anchored heavy chain and a light chain (beta-2 microglobulin). HLA class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells. HLA-B7 is an HLA-B serotype. B7, previously HL-A7, is a serotype that identifies the more common HLA-B*07 gene products and was one of the first 'HL-A' antigens recognized. B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.
References
Product Information
FormatPhycoerythrin
Control
  • Widely expressed
PresentationPurified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate is validated for use in FC for the detection of HLA-B7.
Key Applications
  • Flow Cytometry
Application NotesFlow cytometry - Use 10 μL direct from the vial per 100 μL of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μL buffer.

Optimal working concentrations must be determined by the end user.
Biological Information
ImmunogenPapain solubilized HLA-B7 antigen1
CloneBB7.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityBinds to the HLA-B7 antigen. It does not bind to HLA-B27 or other related HLA antigens.

Cell type Reactivity
Homozygous HLA-B7 ++
Heterozygous HLA-B7 +
Heterozygous HLA-B27 -
Non-HLA-B7, B27 -
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Gene Symbol
  • HLA-B
  • Bw-54
  • Bw-45
  • Bw-47
  • Bw-46
  • Bw-52
  • B-5
  • Bw-4
  • Bw-57
  • OTTHUMP00000034826
  • AS
  • Bw-56
  • Bw-55
  • Bw-41
  • Bw-48
  • B'DT
  • Bw-50
  • Bw-60
  • Bw-58
  • B-21
  • HLA-B-5501
  • B-12
  • Bw-22
  • Bw-53
  • HLAB
  • SPDA1
  • Bw-44
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P30498 # Involved in the presentation of foreign antigens to the immune system.| Q31612 # Involved in the presentation of foreign antigens to the immune system.| P30488 # Involved in the presentation of foreign antigens to the immune system.| P03989 # Involved in the presentation of foreign antigens to the immune system.| P30493 # Involved in the presentation of foreign antigens to the immune system.| P30460 # Involved in the presentation of foreign antigens to the immune system.| P30484 # Involved in the presentation of foreign antigens to the immune system.| P01889 # Involved in the presentation of foreign antigens to the immune system.| P30485 # Involved in the presentation of foreign antigens to the immune system.| Q29940 # Involved in the presentation of foreign antigens to the immune system.| P30483 # Involved in the presentation of foreign antigens to the immune system.| P30495 # Involved in the presentation of foreign antigens to the immune system.| P30492 # Involved in the presentation of foreign antigens to the immune system.| P18465 # Involved in the presentation of foreign antigens to the immune system.| P30487 # Involved in the presentation of foreign antigens to the immune system.| P30480 # Involved in the presentation of foreign antigens to the immune system.| P30491 # Involved in the presentation of foreign antigens to the immune system.| Q95365 # Involved in the presentation of foreign antigens to the immune system.| Q29836 # Involved in the presentation of foreign antigens to the immune system.| Q29718 # Involved in the presentation of foreign antigens to the immune system.| P30475 # Involved in the presentation of foreign antigens to the immune system.| P18464 # Involved in the presentation of foreign antigens to the immune system.| P30479 # Involved in the presentation of foreign antigens to the immune system.| P30490 # Involved in the presentation of foreign antigens to the immune system.| P10319 # Involved in the presentation of foreign antigens to the immune system.| P30466 # Involved in the presentation of foreign antigens to the immune system.| P30461 # Involved in the presentation of foreign antigens to the immune system.| P18463 # Involved in the presentation of foreign antigens to the immune system.| Q04826 # Involved in the presentation of foreign antigens to the immune system.| P30481 # Involved in the presentation of foreign antigens to the immune system.| P30685 # Involved in the presentation of foreign antigens to the immune system.| P30462 # Involved in the presentation of foreign antigens to the immune system.| Q31610 # Involved in the presentation of foreign antigens to the immune system.| P30486 # Involved in the presentation of foreign antigens to the immune system.| P30464 # Involved in the presentation of foreign antigens to the immune system.


SIZE: 362 amino acids; 40478 Da

SUBUNIT: Heterodimer of an alpha chain and a beta chain (beta-2- microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.



DOMAIN: SwissProt: P30498

PTM: Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).

DISEASE: "SwissProt: P03989 # HLA-B27 is associated with the development of ankylosing spondylitis (AS) [MIM:106300]. AS is a chronic inflammatory rheumatic disease that mainly affects the axial skeleton and is considered the prototype of seronegative spondyloarthropathies (SNSA), which include reactive arthritis (e.g., Reiter's syndrome), psoritic spondylitis, and other spondyloarthropathies (SpA). In the Greek Cypriot population, a restricted number of HLA-B27 subtypes are associated with AS and other B27-related diseases and an elevated frequency of the B*2702 allele in the AS patients is identified. The allele B*2707 seems to have a protective role in this population because it was found only in the healthy controls.| P18464 # HLA-B51 is associated with Behcet disease but it is not certain whether HLA-B51 itself or a closely linked gene is responsible for susceptibility. About 20% of healthy individuals of various ethnic origin carries the HLA-B 51 locus, compared with 50 to 80% of patients. Behcet disease is a systemic disorder of recurrent acute inflammation characterized by 4 major symptoms: oral aphthous ulcers, skin lesions, ocular symptoms and genital ulcerations. Occasionally, inflammation in tissues and organs throughout the body can occur, including the gastrointestinal tract, central nervous system, vascular system, lungs, and kidneys. The etiology of Behcet disease is unclear.| P30464 # Involved in susceptibility to Stevens-Johnson syndrome [MIM:608579]. This is a life-threatening reaction of the skin to particular types of medication. It is characterized by high fever, malaise and blistering exanthema. The more severe form of the disease is referred to as toxic epidermal necrolysis."

SIMILARITY: SwissProt: P30498 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31612 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30488 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P03989 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30493 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30460 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30484 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P01889 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30485 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29940 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30483 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30495 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30492 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18465 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30487 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30480 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30491 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q95365 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29836 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29718 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30475 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30479 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30490 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P10319 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30466 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30461 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18463 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q04826 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30481 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30685 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30462 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31610 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30486 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1288 04053252668111

Documentation

Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate Certificates of Analysis

TitleLot Number
ANTI HUMAN HLA-B7, MONOCLONAL ANTIBODY R-PE CONJUGATED, READY TO USE -
Anti-HLA-B7, clone BB7.1, PE Conjugate 3012087
Anti-HLA-B7, clone BB7.1, PE Conjugate - 2142170 2142170
Anti-HLA-B7, clone BB7.1, PE Conjugate - 2393883 2393883
Anti-HLA-B7, clone BB7.1, PE Conjugate - 2410241 2410241
Anti-HLA-B7, clone BB7.1, PE Conjugate - 2460196 2460196
Anti-HLA-B7, clone BB7.1, -2580660 2580660
Anti-HLA-B7, clone BB7.1, -2638875 2638875
Anti-HLA-B7, clone BB7.1, -2683942 2683942
Anti-HLA-B7, clone BB7.1, -2688628 2688628
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References

Reference overviewPub Med ID
Monoclonal antibodies for analysis of the HLA system.
Brodsky, F M, et al.
Immunol. Rev., 47: 3-61 (1979)  1979

95015 95015