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04-855 Anti-GluR1 Antibody, clone C3T, rabbit monoclonal

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04-855
100 µL  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, ChpIHC, IP, WBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-855
Replaces05-855
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-GluR1 Antibody, clone C3T, rabbit monoclonal
Background InformationL glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.
Product Information
FormatCulture Supernatant
Control
  • Routinely evaluated by immunoblot on rat brain microsomal preparation (12-144)
PresentationCultured supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-GluR1 Antibody, clone C3T detects level of GluR1 & has been published & validated for use in IH, IP & WB.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation: 2 μL of a previous lot immunoprecipitated GluR1 from rat brain microsomal preparation.

Immunohistochemistry: A previous lot of this antibody has been shown to detect GluR1 in human, mouse and rat tissues.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 858-868 (..TLPRNSGAGAS..) of human GluR1 (amino acids 840-850 of mature human GluR1)
CloneC3T
HostRabbit
SpecificityRecognizes GluR1, Mr 106 kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chimpanzee
Species Reactivity NoteHuman. Mouse. Rat. Predicted to cross-react with Chimpanzee based pm 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • GRIA1
  • GluR-A
  • GluR-1
  • GLUR1
  • GluR-K1
  • GLURA
  • HBGR1
  • GLUH1
  • MGC133252
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42261 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 906 amino acids; 101506 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).
TISSUE SPECIFICITY: Widely expressed in brain.
PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
SIMILARITY: SwissProt: P42261 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
Molecular Weight106 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western blotting of rat brain microsomal preparation.

Western Blotting Analysis (WB): A 1:5,000-1:10,000 dilution of this lot detected GluR1 in rat brain microsomal preparation (Catalog # 12-144).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
04-855 04053252623066