Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1212 Anti-Geminin Antibody, clone 4B10.1

Detect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting.

Anti-Geminin Antibody, clone 4B10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: GMNN UniProt Number: O75496
View Products on Sigmaaldrich.com
MABS1212
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1212
DescriptionAnti-Geminin Antibody, clone 4B10.1
Alternate Names
  • Geminin
Background InformationGeminin (UniProt O75496) is encoded by the GMNN gene (Gene ID 51053) in human. Geminin is a negative regulator of DNA-replication and the founding member of the Geminin family, whose members also include Idas (multicilin; McIdas) and GemC1 (Geminin coiled-coil domain-containing protein 1). Geminin binds Cdt1 and inhibits the mini-chromosome maintenance complex (MCM) loading onto chromatin and pre-replication complex (preRC) formation. Geminin is a 209-amino acid protein that can form homodimer or heterodimers with either Idas or GemC1 via the middle coiled coil structure (a.a. 94-144 of Geminin) in a head-to-tail fashion. The same region in Geminin (a.a. 82-161) is also essential for Cdt1 interaction. A homeodomain-binding domain is located at the C-terminal region of Geminin (a.a. 170-190). GemC1 is also implicated in DNA replication but at a later stage than Geminin. All three Geminin family members play important roles in cell proliferation and differentiation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear geminin immunoreactivity in HUVECs, A431, and HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant human geminin internal fragment.
Clone4B10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4B10.1 detects human geminin by targeting an internal epitope.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GMNN
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~31 kDa observed. 23.57 kDa calculated. The larger-than-predicted band size is consistent with that reported in the literature (McGarry, T.J., and Kirschner, M.W. (1998). Cell. 93(6):1043-1053 ). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected geminin in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1212 04054839049736