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MABC1097-100UG Anti-GEF-H1 Antibody, clone 14B11

Anti-GEF-H1, clone 14B11, Cat. No. MABC1097, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1) and has been tested for use in Western Blotting.

MABC1097-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1097-100UG
DescriptionAnti-GEF-H1 Antibody, clone 14B11
Alternate Names
  • Rho guanine nucleotide exchange factor 2
  • Guanine nucleotide exchange factor H1
  • Microtubule-regulated Rho-GEF
  • Proliferating cell nucleolar antigen p40
Background InformationRho guanine nucleotide exchange factor 2 (UniProt: Q92974; also known as Guanine nucleotide exchange factor H1, GEF-H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40) is encoded by the ARHGEF2 (also known as KIAA0651, LFP40) gene (Gene ID: 9181) in human. GEF-H1 is a microtubule-associated guanine nucleotide exchange factor (GEF) for the Rho family of small GTPases that activates Rho-GTPases by promoting the exchange of GDP for GTP. It localizes to the tips of cortical microtubules of the mitotic spindle during cell division and is released upon microtubule depolymerization. GEF-H1 is involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and innate immune response. GEF-H1 can act as an adaptor protein that links PP2A B subunits to KSR-1 and mediate the dephosphorylation of KSR-1 serine 392 and activate MAPK signaling. GEF-H1 I known to contributes to cell survival and growth in HRASV12- transformed cells and is reported to be essential for activation of MAP kinase pathway in response to HRASV12. It is also reported to be important for the growth and survival of HRASV12 transformed cells. GEF-H1 contains a DH (DBL-homology) domain (aa 235-432) that interacts with and promotes loading of GTP on RhoA and tyrosine phosphorylation of RIPK2. It also has two phorbol-ester/DAG-type zinc finger (aa 587-611) and the C-terminal coiled coil domains (aa 798-867), both of which are required for association with microtubules. (Ref.: Cullis, J., et al. (2014). Cancer Cell 25(2); 181-195).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-GEF-H1, clone 14B11, Cat. No. MABC1097, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1) and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected GEF-H1 in Western Blotting applications (Cullis, J., et. al. (2014). Cancer Cell. 25(2):181-95).
Biological Information
ImmunogenLinear peptide from C-terminal region of human GEF-H1.
Clone14B11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14B11 is a mouse monoclonal antibody that specifically detects human Rho guanine nucleotide exchange factor 2 (GEF-H1).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARHGEF2
  • KIAA0651
  • LFP40
Purification MethodProtein G purified
UniProt Number
Molecular Weight~111 kDa observed; 111.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from untreated HCT116 cells and those pretreated with MEK1/2 inhibitors PD98059 and U0126.

Western Blotting Analysis: 2 µg/mLof this antibody detected GEF-H1 in untreated HCT116 cells, but not in cells pretreated with MEK1/2 inhibitors PD98059 and U0126.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1097-100UG 04054839997402

Documentation

Anti-GEF-H1 Antibody, clone 14B11 SDS

Title

Safety Data Sheet (SDS) 

Anti-GEF-H1 Antibody, clone 14B11 Certificates of Analysis

TitleLot Number
Anti-GEF-H1, clone 14B11 - Q3239908 Q3239908

References

Reference overviewPub Med ID
The RhoGEF GEF-H1 is required for oncogenic RAS signaling via KSR-1.
Cullis, J; Meiri, D; Sandi, MJ; Radulovich, N; Kent, OA; Medrano, M; Mokady, D; Normand, J; Larose, J; Marcotte, R; Marshall, CB; Ikura, M; Ketela, T; Moffat, J; Neel, BG; Gingras, AC; Tsao, MS; Rottapel, R
Cancer Cell  28  181-98  2017

Show Abstract 		24525234 24525234

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