Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB5406B Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate

This Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: GAD1, GAD, GAD67 UniProt Number: Q99259
View Products on Sigmaaldrich.com
MAB5406B
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICC, IHCMBiotinMonoclonal Antibody
Description
Catalogue NumberMAB5406B
DescriptionAnti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
Alternate Names
  • Glutamate decarboxylase 1
  • 67 kDa glutamic acid decarboxylase
  • GAD-67
  • Glutamate decarboxylase 67 kDa isoform
Background InformationGutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
References
Product Information
FormatBiotin
Control
  • Rat cortex tissue
PresentationPurified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells.
Biological Information
ImmunogenRecombinant GAD67 protein
Clone1G10.2
HostMouse
SpecificityReacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate.
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAD1
  • GAD
  • GAD67
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Catalyzes the production of GABA.

CATALYTIC ACTIVITY: L-glutamate = 4-aminobutanoate + CO2.

COFACTOR: Pyridoxal phosphate.

SUBUNIT STRUCTURE: Homodimer.

TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

INVOLVEMENT IN DISEASE: Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

SEQUENCE SIMILARITIES: Belongs to the group II decarboxylase family.
Molecular Weight~67 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in rat cortex tissue.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5406B 04053252647765

Documentation

Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate Certificates of Analysis

TitleLot Number
Anti-GAD67, clone 1G10.2, Biotin - 3218869 3218869
Anti-GAD67, clone 1G10.2, Biotin - 3244044 3244044
Anti-GAD67, clone 1G10.2, Biotin - 3482212 3482212
Anti-GAD67, clone 1G10.2, Biotin - 3660430 3660430
Anti-GAD67, clone 1G10.2, Biotin - 3773132 3773132
Anti-GAD67, clone 1G10.2, Biotin - 3786050 3786050
Anti-GAD67, clone 1G10.2, Biotin - 3807971 3807971
Anti-GAD67, clone 1G10.2, Biotin - 3876259 3876259
Anti-GAD67, clone 1G10.2, Biotin - 3906986 3906986
Anti-GAD67, clone 1G10.2, Biotin - 4047586 4047586
Show more