Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC524 Anti-FANCC Antibody, clone 8F3

This Anti-FANCC Antibody, clone 8F3 is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of FANCC.

Anti-FANCC Antibody, clone 8F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FANCC, FAC, FACC UniProt Number: Q00597
View Products on Sigmaaldrich.com
MABC524
200 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, HWB, IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC524
DescriptionAnti-FANCC Antibody, clone 8F3
Alternate Names
  • Fanconi anemia group C protein
  • Protein FACC
  • FANCC
Background InformationFanconi anemia (FA) is an inherited bone marrow failure syndrome associated with a progressive decline in hematopoietic stem cells, developmental defects, and predisposition to cancer. FANCC is part of the multisubunit Fanconi anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is a primarily nuclear localized DNA repair protein that is believed to function in interstrand DNA cross-link repair and the maintenance of normal chromosome stability. Mutations in FANCC are associated with various aspects of FA. New research shows that FANCC is critical for resistance to DNA cross-linking drug-induced DNA damage in cells patients with myeloproliferative neoplasms. Also, FANCC interacts with the C-terminal-binding protein-1 (CtBP1) involved in transcriptional regulation, including being a negative regulator of Dickkopf-1 (DKK1) expression. This down regulation does not occur in mutations of FANCC, which then leads to up-regulation of DKK1, a molecule involved in hematopoietic malignancies.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-FANCC Antibody, clone 8F3 is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of FANCC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 4 µg/mL of a representative lot of this antibody detected FANCC in 10 µg of Mouse brain lysate.

Western Blotting Analysis: A representative lot detected FANCC in HeLa cell lysate (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FANCC from HeLa cell lysate (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).

Immunocytochemistry Analysis: A representative lot detected FANCC in HeLa cells (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).
Biological Information
ImmunogenLinear peptide corresponding to Human FANCC.
Clone8F3
Concentration1 mg/mL
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Rat
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FANCC
  • FAC
  • FACC
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 63 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected FANCC in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC524 04053252983597