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PC168 Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb

This Anti-Estrogen Receptor β Rabbit pAb is validated for use in Frozen Sections, Gel Shift, WB, Paraffin Sections for the detection of Estrogen Receptor β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ER β

PC168
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody
Description
OverviewRecognizes the ~60 kDa estrogen receptor β protein in overexpressing cell lines and breast carcinoma tissue. Does not cross-react with estrogen receptor α.
Catalogue NumberPC168
Brand Family Calbiochem®
SynonymsAnti-ER β
Application Data
Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
References
ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinology 66, 63.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem. J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Positive controlBreast carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (5-10 µg/ml)
Gel Shift (1-5 µl)
Immunoblotting (1 µg/ml)
Paraffin Sections (1:50)
Application CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC168 0

Documentation

Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb Certificates of Analysis

TitleLot Number
PC168

References

Reference overview
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinology 66, 63.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem. J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-October-2007 RFH
SynonymsAnti-ER β
ApplicationFrozen Sections (5-10 µg/ml)
Gel Shift (1-5 µl)
Immunoblotting (1 µg/ml)
Paraffin Sections (1:50)
Application Data
Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa estrogen receptor beta protein.
BackgroundSteroids regulate important neuronal functions including the organization of neural circuits during development and the regulation of synapse organization in the adult. Steroid hormones may promote cell survival and differentiation of both neurons and glial cells, regulate synaptogenesis, prevent synapse elimination, cause axonal and dendritic growth, and play a role during regeneration of the nervous system. Steroid hormone receptors are members of a large family of nuclear ligand-activated transcription factors that includes the androgen, estrogen, glucocorticoid, and progesterone receptors. In the absence of hormone, steroid receptors are associated in complexes with heat shock proteins and immunophilins. Steroid binding results in the dissociation of the heat-shock protein, receptor dimerization, and binding to specific hormone response elements (HRE) to produce a transcriptionally active complex that leads to the activation or repression of target genes. Steroid receptors are regulated by phosphorylation, however, modulation of kinase activity can also cause receptor activation in the absence of hormone. Recent evidence suggests that steroid receptor expression can be regulated by neurotrophins, and that steroids can regulate the expression of trophic factors and their receptors. Estrogen receptor Beta has been shown to be a high affinity estrogen binding protein capable of initiating transcription of genes under the control of estrogen response elements. RNA distribution studies have shown that estrogen receptor beta is transcribed in human thymus, spleen, ovary and testis as well as rat ovary and prostate.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
IsotypeIgG
Speciesmouse, rat
Positive controlBreast carcinoma tissue
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinology 66, 63.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem. J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.