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MAB4444 Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8

This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation, Adhesion (CAMs) Gene Symbol: EPCAM, 17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, Ly74, M1S2, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2 UniProt Number: P16422
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MAB4444
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4444
DescriptionAnti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8
Alternate Names
  • Adenocarcinoma-associated antigen
  • CD326 antigen
  • Cell surface glycoprotein Trop-1
  • Epithelial cell surface antigen
  • Epithelial glycoprotein
  • KS 1/4 antigen
  • Major gastrointestinal tumor-associated protein GA733-2
  • antigen identified by monoclonal antibody AUA1
  • carcinoma-associated antigen GA733-2
  • epithelial cell adhesion molecule
  • human epithelial glycoprotein-2
  • membrane component, chromosome 4, surface marker (35kD glycoprotein)
  • tumor-associated calcium signal transducer 1
Background InformationEpithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.
References
Product Information
FormatPurified
Control
  • Human embryonic stem cells
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blotting
Application NotesFlow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
Biological Information
ImmunogenHES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)
EpitopeUnknown
Clonemab 8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq].
Gene Symbol
  • EPCAM
  • 17-1A
  • 323/A3
  • CD326
  • CO-17A
  • CO17-1A
  • EGP
  • EGP-2
  • EGP34
  • EGP40
  • ESA
  • Ep-CAM
  • GA733-2
  • HEA125
  • KS1/4
  • KSA
  • Ly74
  • M1S2
  • M4S1
  • MH99
  • MIC18
  • MK-1
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • hEGP-2
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection.

SUBUNIT STRUCTURE: Monomer.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

TISSUE SPECIFICTY: This protein is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas.

SEQUENCE SIMILARITIES: Belongs to the EPCAM family.

Contains 1 thyroglobulin type-1 domain.
Molecular Weight~ 40 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in human embryonic stem cells.

Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4444 04053252617447

Documentation

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 SDS

Title

Safety Data Sheet (SDS) 

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 Certificates of Analysis

TitleLot Number
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 (KC) Monoclonal Antibody 2957938
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2145979 2145979
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2397157 2397157
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2017301 2017301
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2088984 2088984
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2291714 2291714
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2561012 2561012
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3204853 3204853
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3698151 3698151
Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3833724 3833724
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