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MABS815 Anti-ERK3, clone 4E8 Antibody

Anti-ERK3, clone 4E8 Antibody is an antibody against ERK3 for use in Western Blotting, Flow Cytometry, ELISA.

Anti-ERK3, clone 4E8 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAPK6, ERK3, PRKM6 UniProt Number: Q16659
MABS815
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS815
ReplacesMABS169
DescriptionAnti-ERK3, clone 4E8 Antibody
Alternate Names
  • Mitogen-activated protein kinase 6
  • MAP kinase 6
  • MAPK 6
  • ERK3
Background InformationERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop. Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells. Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells. ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-ERK3, clone 4E8 Antibody is an antibody against ERK3 for use in Western Blotting, Flow Cytometry, ELISA.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • ELISA
Application NotesFlow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected ERK3 in HeLa cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human ERK3 expressed in E. Coli. 
Clone4E8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MAPK6
  • ERK3
  • PRKM6
Purification MethodUnpurified
UniProt Number
Molecular Weight~54 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 and ERK3-hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected ERK3 in transfected HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS815 04055977172331