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CA1019 Anti-EPLIN, Internal Rabbit pAb

Anti-EPLIN, Internal Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epithelial Protein Lost in Neoplasm

CA1019
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~67 kDa α and the ~85 kDa β isoforms of EPLIN in OU145 cells.
Catalogue NumberCA1019
Brand Family Calbiochem®
SynonymsAnti-Epithelial Protein Lost in Neoplasm
Application Data
Detection of human EPLIN by immunoprecipitation. Sample: Whole cell lysate from DU145 cells. Antibody for immunoprecipitation: Anti-EPLIN, Internal Rabbit pAb (Cat. No. CA1019) (10 µg/mg total protein). Antibody for immunoblotting: Anti-EPLIN C-Terminal Rabbit pAb (Cat. No. CA1014) (1:1000). Detection: chemiluminescence.
References
ReferencesMaul, R.S., et al. 2003. J. Cell Biol. 160, 399.
Chen, S., et al. 2000. Gene 248, 69.
Maul, R.S., et al. 1999. Oncogene 18, 7838.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlDU145 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Not Western Blot
Application NotesImmunoprecipitation (10 µg/mg total protein)
Immunoblotting (not recommended, see comments)
Application CommentsFor immunoblotting, Anti-EPLIN, Human (Rabbit), Cat. No. CA1014 is recommended. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from an internal region of human EPLIN
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CA1019 0

Documentation

References

Reference overview
Maul, R.S., et al. 2003. J. Cell Biol. 160, 399.
Chen, S., et al. 2000. Gene 248, 69.
Maul, R.S., et al. 1999. Oncogene 18, 7838.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Epithelial Protein Lost in Neoplasm
ApplicationImmunoprecipitation (10 µg/mg total protein)
Immunoblotting (not recommended, see comments)
Application Data
Detection of human EPLIN by immunoprecipitation. Sample: Whole cell lysate from DU145 cells. Antibody for immunoprecipitation: Anti-EPLIN, Internal Rabbit pAb (Cat. No. CA1019) (10 µg/mg total protein). Antibody for immunoblotting: Anti-EPLIN C-Terminal Rabbit pAb (Cat. No. CA1014) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~67 kDa α and the ~85 kDa β isoforms of EPLIN.
BackgroundEPLIN (Epithelial Protein Lost in Neoplasm, Accesion No. NP 057441) was originally identified as the product of a gene that is down-regulated in prostate and breast cancer cell lines. In humans, EPLIN is expressed as two isoforms, EPLIN-α (~67 kDa) and EPLIN-β (~85 kDa), as a result of alternative promoter usage from a single gene on chromosome 12. The EPLIN-β isoform differs only from the EPLIN-α isoform by an additional 160 amino acids at the N-terminus. EPLIN-α expression is preferentially down-regulated in transformed cells. EPLIN contains a LIM domain that may facilitate dimerization and protein association. Expression of EPLIN increases the assembly of cellular stress fibers and inhibits membrane ruffling induced by active Rac. EPLIN stabilizes preformed filaments through its ability to interact with and bundle actin filaments. Loss of EPLIN may contribute to transformation due to rapid turnover of filaments associated with motility.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from an internal region of human EPLIN
IsotypeIgG
Specieshuman
Positive controlDU145 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor immunoblotting, Anti-EPLIN, Human (Rabbit), Cat. No. CA1014 is recommended. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
ReferencesMaul, R.S., et al. 2003. J. Cell Biol. 160, 399.
Chen, S., et al. 2000. Gene 248, 69.
Maul, R.S., et al. 1999. Oncogene 18, 7838.