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ABS2237-100UG Anti-EFCAB9

Anti-EFCAB9, Cat. No. ABS2237, is a rabbit polyclonal antibody that detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9) and is tested for use in Immunocytochemistry and Western Blotting.

ABS2237-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2237-100UG
DescriptionAnti-EFCAB9
Alternate Names
  • EF-hand calcium-binding domain-containing protein 9
Background InformationEF-hand calcium-binding domain-containing protein 9 (UniProt: Q9DAM2; also known as EFCAB9) is encoded by the Efcab9 gene (Gene ID: 69306) in murine species. EFCAB9 is an evolutionarily conserved, testis-specific protein that is expressed after meiosis. It serves as a bifunctional cytoplasmic machine that modulates the channel activity and the domain organization of CatSper. It is a pH-dependent calcium sensor that is required for activation of CatSper channel required for sperm cell hyperactivation. This hyperactivation is essential for sperm motility and fertilization. It associates with the CatSper complex via direct interaction with subunit CATSPERz, and senses intracellular Calcium levels. However, it dissociates at elevated pH. It is shown that EFCAB9-CatSperz protein pairs interact with the channel pore as a gatekeeper before capacitation, but it activates the channel in response to capacitation-associated intracellular pH and changes in calcium levels. ECFCAB9 is reported to contain three conserved EF-hand Calcium binding domains. Mice deficient in EFCAB9 display sperm motility defects and compromised fertility. (Ref.: Hwang, JY., et al., (2019). 177(6); 1480-1494).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 10 mM HEPES, 140 mM NaCl, 50% glycerol, pH 7.5, with 50% glycerol.
Quality LevelMQ200
Applications
ApplicationAnti-EFCAB9, Cat. No. ABS2237, is a rabbit polyclonal antibody that detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9) and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot detected EFCAB9 in Immunocytochemistry applications (Hwang, J.Y., et. al. (2019). Cell. 177(6):1480-1494).

Western Blotting Analysis: A representative lot detected EFCAB9 in Western Blotting applications (Hwang, J.Y., et. al. (2019). Cell. 177(6):1480-1494).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EFCAB9 in mouse sperm lysate, but not in Efcab9 knockout mouse sperm lysate (Courtesy of Dr. Jean-Ju Chung @ Yale University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the EF-hand 3, C-terminal half of mouse EF-hand calcium-binding domain-containing protein 9 (EFCAB9).
EpitopeC-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9). It targets an epitope within 18 amino acids from the EF-hand 3 in the C-terminal half.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Efcab9
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~23 kDa observed; 26.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse sperm lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EF-hand calcium-binding domain-containing protein 9 (EFCAB9) in wild-type, but not in EFCAB9 knockout mouse sperm lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2237-100UG 04065265398138