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ST1703 Anti-DLL1 Mouse mAb (4F8)

Anti-DLL1, mouse monoclonal, clone 4F8, recognizes the DLL1 protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-DLL1 Mouse mAb (4F8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Delta-like 1

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the DLL1 protein in HeLa cells.
Catalogue NumberST1703
Brand Family Calbiochem®
SynonymsAnti-Delta-like 1
Application Data
Detection of human DLL1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human DLL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (10 µg/ml). Detection: fluorescence.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µ/g/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 18-110 human DLL1, expressed as a GST fusion protein
Clone4F8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1703 0

Documentation

Anti-DLL1 Mouse mAb (4F8) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-July-2012 JSW
SynonymsAnti-Delta-like 1
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human DLL1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human DLL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the DLL1 protein.
BackgroundThe DLL1 protein is a human homolog of the Notch Δ ligand and is a member of the δ/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may also play a role in cell-to-cell communication.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 18-110 human DLL1, expressed as a GST fusion protein
Clone4F8
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µ/g/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review