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AB4330 Anti-DDX4 (Vasa) Antibody

Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).

Anti-DDX4 (Vasa) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation, RNA Metabolism & Binding Proteins Gene Symbol: VASA, MVH, DDX4 UniProt Number: Q61496
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AB4330
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB4330
DescriptionAnti-DDX4 (Vasa) Antibody
Alternate Names
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • probable ATP-dependent RNA helicase DDX4
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
  • Vasa homolog
  • DEAD box protein 4
Background InformationDDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.
References
Product Information
FormatAffinity Purified
Control
  • Mouse testis tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).
EpitopeC-terminus
HostRabbit
SpecificityThis antibody recognizes DDX4 (Vasa) at the C-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VASA
  • MVH
  • DDX4
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May play a role in germ cell development.

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Ref.3 SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › perinuclear region.

TISSUE SPECIFICTY: Testis.

DEVELOPMENTAL STAGE: Expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage.

SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.
Molecular Weight~76 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB4330 04053252526923