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CC12F Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) Fluorescein Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CC12F
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
Catalogue NumberCC12F
Brand Family Calbiochem®
References
ReferencesInaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.
Product Information
FormLiquid
FormulationIn 50% glycerol, 50% sodium phosphate buffer.
Positive controlA431 or MCF7 cells
PreservativeNone
Applications
Application ReferencesImmunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949. Bartkova, J., et al. 1994. J. Pathol. 172, 237. Lukas, J., et al. 1994. Oncogene 9, 707.
Key Applications Flow Cytometry
Immunofluorescence
Application NotesFlow Cytometry (5 µg/ml)
Immunofluorescence (5 µg/ml)
Application CommentsDoes not react with cyclins D2 or D3. For immunoblotting, immunohistochemistry, immunoprecipitation and neutralization studies, we recommend Cat. No. CC12. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human cyclin D1
ImmunogenHuman
CloneDCS-6
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CC12F 0

Documentation

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) Fluorescein Conjugate Certificates of Analysis

TitleLot Number
CC12F

References

Reference overview
Inaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
ApplicationFlow Cytometry (5 µg/ml)
Immunofluorescence (5 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
BackgroundCyclins are a family of proteins implicated in the induction and control of mitosis. They are characterized by their accumulation and dramatic destruction in a cell cycle dependent manner. Cyclins are necessary for cdc/cdk kinase activity and are one of the subunits of the active kinase, in association with cdc2 or one of the cdk family members. Several different major cyclin families have been identified to date. These include the A, B, D and E family of cyclins. The D family of cyclins has been associated with a wide variety of proliferative diseases but their biochemical role is still unknown. The D cyclins appear in early G1, before the B cyclins. The human cyclin D1 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product. PRAD1 was originally identified as being a clonally rearranged and overexpressed gene linked to the bcl-1 locus in a subset of benign parathyroid tumors. Amplification of cyclin D1 genes has also been demonstrated in human esophageal cancer.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant human cyclin D1
CloneDCS-6
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlA431 or MCF7 cells
FormLiquid
FormulationIn 50% glycerol, 50% sodium phosphate buffer.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsDoes not react with cyclins D2 or D3. For immunoblotting, immunohistochemistry, immunoprecipitation and neutralization studies, we recommend Cat. No. CC12. Antibody should be titrated for optimal results in individual systems.
Storage Protect from light
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesInaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.
Application referencesImmunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949. Bartkova, J., et al. 1994. J. Pathol. 172, 237. Lukas, J., et al. 1994. Oncogene 9, 707.