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MABC554 Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1

This Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 is validated for use in Western Blotting for the detection of Cullin-4A/CUL4A.

Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CUL4A UniProt Number: Q13619
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MABC554
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC554
DescriptionAnti-Cullin-4A/CUL4A Antibody, clone 2A2.1
Alternate Names
  • Cullin-4A
  • CUL-4A
Background InformationCullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. Cullin-4 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The human genome encodes 8 cullin proteins (CUL 1, 2, 3, 4A, 4B, 5, 7 and 9). The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). CUL4A interacts with the substrate-binding adaptor DDB1 (DNA damage binding protein 1) via its arc-shaped helical N-terminal domain, and binds the small RING finger protein ROC1 (regulator of cullins 1) via its globular C-terminal domain. Despite the 83% sequence identity and seemingly functional redundancy between CUL4A and CUL4B, much more focus is placed on CUL4A in oncogenesis research.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 is validated for use in Western Blotting for the detection of Cullin-4A/CUL4A.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Cullin-4A/CUL4A near the C-terminus.
EpitopeNear C-terminus
Clone2A2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2A2.1 is expected to react with both spliced isoforms of human cullin-4A
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CUL4A
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~88 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human melanoma SK-MEL-28 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-4A/CUL4A in 10 µg of human melanoma SK-MEL-28 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC554 04055977288308