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AB3830 Anti-Caveolin-1 Antibody, phospho-specific (Tyr14)

This Anti-Caveolin-1 Antibody, phospho-specific (Tyr14) is validated for use in IP, WB for the detection of Caveolin-1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: CAV1, MSTP085, CAV, VIP21, Caveolin-1. UniProt Number: Q03135
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AB3830
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Mk, RIP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3830
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Caveolin-1 Antibody, phospho-specific (Tyr14)
Product Information
FormatAffinity Purified
PresentationProtein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-Caveolin-1 Antibody, phospho-specific (Tyr14) is validated for use in IP, WB for the detection of Caveolin-1.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Immunoprecipitation 1:50

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Tyr14 of human caveolin-1
Epitopephospho-specific (Tyr14)
HostRabbit
SpecificityRecognizes caveolin-1 only when phosphorylated at tyrosine 14. This antibody does not cross-react with caveolin-2, -3 or caveolin-1beta, or the short isoform of caveolin-1.
Species Reactivity
  • Human
  • Mouse
  • Monkey
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.
Gene Symbol
  • CAV1
  • MSTP085
  • CAV
  • VIP21
  • Caveolin-1.
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q03135 # May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity).
SIZE: 178 amino acids; 20472 Da
SUBUNIT: Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane, caveola; Peripheral membrane protein; Cytoplasmic side. Note=Membrane protein of caveolae. Potential hairpin-like structure in the membrane.
TISSUE SPECIFICITY: In muscle and lung, less so in liver, brain and kidney.
PTM: The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
SIMILARITY: SwissProt: Q03135 ## Belongs to the caveolin family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20ºC for 12 months, do not aliquot.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
AB3830 04053252338694