Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB10753 Anti-Caspase3 (active form) Antibody, clone 3D9.3

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CASP3, CPP32 UniProt Number: P42574
View Products on Sigmaaldrich.com
MAB10753
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB10753
Replaces04-1090; 04-439
DescriptionAnti-Caspase3 (active form) Antibody, clone 3D9.3
Alternate Names
  • Caspase-3
  • CASP-3
  • Apopain
  • Cysteine protease CPP32
  • CPP-32
  • Protein Yama
  • SREBP cleavage activity 1
  • SCA-1
  • Caspase-3 subunit p17
  • Caspase-3 subunit p12
Background InformationCaspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Caspase3 (active form).
Clone3D9.3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.
IsotypeIgMκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CASP3
  • CPP32
Purification MethodPurified by Ion-Exchange Chromatography
UniProt Number
Molecular Weight~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB10753 04053252850653

Documentation

Anti-Caspase3 (active form) Antibody, clone 3D9.3 SDS

Title

Safety Data Sheet (SDS) 

Anti-Caspase3 (active form) Antibody, clone 3D9.3 Certificates of Analysis

TitleLot Number
Anti-Caspase3 (active form), Clone 3D9.3 - 4068671 4068671
Anti-Caspase3 (active form), clone -2550999 2550999
Anti-Caspase3 (active form), clone -2807042 2807042
Anti-Caspase3 (active form), clone 3D9.3 - 2398965 2398965
Anti-Caspase3 (active form), clone 3D9.3 - Q2116523 Q2116523
Anti-Caspase3 (active form), clone 3D9.3 - 3425550 3425550
Anti-Caspase3 (active form), clone 3D9.3 - 3462119 3462119
Anti-Caspase3 (active form), clone 3D9.3 - 3700473 3700473
Anti-Caspase3 (active form), clone 3D9.3 - 3831721 3831721
Anti-Caspase3 (active form), clone 3D9.3 - 3931722 3931722
Show more