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PC389 Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb

This Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb is validated for use in Immunoblotting for the detection of CXCR4 (Ab-1) (182-196).

Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fusin, Anti-HUMSTR, Anti-LESTR

PC389
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
OverviewRecognizes the ~40 kDa CXCR4 protein in HeLa cells.
Catalogue NumberPC389
Brand Family Calbiochem®
SynonymsAnti-Fusin, Anti-HUMSTR, Anti-LESTR
Application Data
Detection of human CXCR4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb (Cat. No. PC389) (1 μg/ml). Detection: chemiluminescence.
References
ReferencesBrelot, A., et al. 1997. J. Virol. 71, 4744.
Dimitrov, D.S. 1997. Cell 91, 721.
Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
Berson, J.F., et al. 1996. J. Virol. 70, 6288.
Bleul, C.C., et al. 1996. Nature 382, 829.
Doranz, B.J., et al. 1996. Cell 85, 1149.
Endres, M.J., et al. 1996. Cell 87, 745.
Feng, Y., et al. 1996. Science 272, 872.
Lapham, C.K., et al. 1996. Science 274, 602.
Oberlin, E., et al. 1996. Nature 382, 833.
Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunoblotting Phillips, R.J., et al. 2005. J. Biol. Chem. 280, 22473.
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (2-4µg/ml, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 182-196 in the second extracellular loop of human CXCR4
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC389 0

Documentation

Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb Certificates of Analysis

TitleLot Number
PC389

References

Reference overview
Brelot, A., et al. 1997. J. Virol. 71, 4744.
Dimitrov, D.S. 1997. Cell 91, 721.
Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
Berson, J.F., et al. 1996. J. Virol. 70, 6288.
Bleul, C.C., et al. 1996. Nature 382, 829.
Doranz, B.J., et al. 1996. Cell 85, 1149.
Endres, M.J., et al. 1996. Cell 87, 745.
Feng, Y., et al. 1996. Science 272, 872.
Lapham, C.K., et al. 1996. Science 274, 602.
Oberlin, E., et al. 1996. Nature 382, 833.
Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.

Citations

Title
  • Roderick J. Phillips, et al. (2005) Epidermal Growth Factor and Hypoxia-induced Expression of CXC Chemokine Receptor 4 on Non-small Cell Lung Cancer Cells is Regulated by the Phosphatidylinositol 3-Kinase/PTEN/Akt/Mammalian Target of Rapamycin Signaling Pathway and Activation of Hypoxia Ind. Journal of Biological Chemistry 280, 22473-22481.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision10-September-2008 RFH
    SynonymsAnti-Fusin, Anti-HUMSTR, Anti-LESTR
    ApplicationImmunoblotting (2-4µg/ml, see application references)
    Application Data
    Detection of human CXCR4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb (Cat. No. PC389) (1 μg/ml). Detection: chemiluminescence.
    DescriptionPurified rabbit polyclonal antibody. Recognizes the ~40 kDa CXCR4 protein.
    BackgroundHuman immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1, and V28 were recently identified as HIV coreceptors1. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The α-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide corresponding to amino acids 182-196 in the second extracellular loop of human CXCR4
    IsotypeIgG
    Specieshuman
    Positive controlHeLa cells
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBrelot, A., et al. 1997. J. Virol. 71, 4744.
    Dimitrov, D.S. 1997. Cell 91, 721.
    Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
    Berson, J.F., et al. 1996. J. Virol. 70, 6288.
    Bleul, C.C., et al. 1996. Nature 382, 829.
    Doranz, B.J., et al. 1996. Cell 85, 1149.
    Endres, M.J., et al. 1996. Cell 87, 745.
    Feng, Y., et al. 1996. Science 272, 872.
    Lapham, C.K., et al. 1996. Science 274, 602.
    Oberlin, E., et al. 1996. Nature 382, 833.
    Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.
    Citation
  • Roderick J. Phillips, et al. (2005) Epidermal Growth Factor and Hypoxia-induced Expression of CXC Chemokine Receptor 4 on Non-small Cell Lung Cancer Cells is Regulated by the Phosphatidylinositol 3-Kinase/PTEN/Akt/Mammalian Target of Rapamycin Signaling Pathway and Activation of Hypoxia Ind. Journal of Biological Chemistry 280, 22473-22481.
    		•
    Application referencesImmunoblotting Phillips, R.J., et al. 2005. J. Biol. Chem. 280, 22473.