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ST1100 Anti-CRM1 Rabbit pAb

Anti-CRM1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Exportin, Anti-Chromosome region maintenance 1 protein

ST1100
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~100 kDa human CRM1 protein in HeLa cells.
Catalogue NumberST1100
Brand Family Calbiochem®
SynonymsAnti-Exportin, Anti-Chromosome region maintenance 1 protein
Application Data
Lane 1: Detection of human CRM1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells. Primary antibody: Anti-CRM1 Rabbit pAb (Cat. No. ST1100) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human CRM1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HeLa cells. Antibody for IP: Anti-CRM1 Rabbit pAb (ST1100) (5 µg/500 µg total protein in 500 µl). Immunoblotting conditions: same as Lane 1.
References
ReferencesKudo, N., et al. 1998. Exp. Cell Res. 242, 540.
Fornerod, M., et al. 1997. Cell 90, 1051.
Kudo, N., et al. 1997. J. Biol. Chem. 272, 29742.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate bufer, pH 7-8.
Positive controlHeLa cells
Preservative≤ 0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (10 µg/mg total protein/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids in the C terminal region of human CRM1
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1100 0

Documentation

Anti-CRM1 Rabbit pAb Certificates of Analysis

TitleLot Number
ST1100

References

Reference overview
Kudo, N., et al. 1998. Exp. Cell Res. 242, 540.
Fornerod, M., et al. 1997. Cell 90, 1051.
Kudo, N., et al. 1997. J. Biol. Chem. 272, 29742.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2007 RFH
SynonymsAnti-Exportin, Anti-Chromosome region maintenance 1 protein
ApplicationImmunoblotting (1 µg/ml)
Immunoprecipitation (10 µg/mg total protein/ml)
Application Data
Lane 1: Detection of human CRM1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells. Primary antibody: Anti-CRM1 Rabbit pAb (Cat. No. ST1100) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human CRM1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HeLa cells. Antibody for IP: Anti-CRM1 Rabbit pAb (ST1100) (5 µg/500 µg total protein in 500 µl). Immunoblotting conditions: same as Lane 1.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~100 kDa CRM1 protein.
BackgroundCRM1 (accession #NP003391) is a member of the karyopherin family of nucleocytoplasmic transport factors. It interacts with a wide range of nuclear proteins that contain leucine-rich nuclear export signals (NES) and transports these proteins to the cytoplasm via the nuclear pore complex (NPC). Similar to other karyopherins involved in nuclear export, CRM1 binds to its cargo molecules in the nucleus in the presence of activated Ran (GTPase). Following nuclear export, hydrolysis of bound GTP to GDP results in a conformational change in Ran that leads to release of the cargo molecule from CRM1 in the cytoplasm, thus providing directionality to the export pathway. CRM1 also interacts with components of the NPC, which is the portal used for all nucleocytoplasmic transport. This interaction is essential for CRM1-mediated export. Leptomycin B is a Streptomyces sp. metabolite that inhibits nuclear export at low nanomolar concentrations by binding directly to and inhibiting the function of CRM1, resulting in its accumulation in the nucleus.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids in the C terminal region of human CRM1
IsotypeIgG
SpeciesXenopus, human
Positive controlHeLa cells
FormLiquid
FormulationIn Tris-citrate/phosphate bufer, pH 7-8.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤ 0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKudo, N., et al. 1998. Exp. Cell Res. 242, 540.
Fornerod, M., et al. 1997. Cell 90, 1051.
Kudo, N., et al. 1997. J. Biol. Chem. 272, 29742.