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MABE628 Anti-CHUK, clone 3G12H9 Antibody

Anti-CHUK, clone 3G12H9 Antibody is an antibody against CHUK for use in Western Blotting, Immunocytochemistry.

Anti-CHUK, clone 3G12H9 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: CHUK, IKKA, TCF16 UniProt Number: O15111
MABE628
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABE628
Replaces04-365
DescriptionAnti-CHUK, clone 3G12H9 Antibody
Alternate Names
  • Inhibitor of nuclear factor kappa-B kinase subunit alpha
  • I-kappa-B kinase alpha
  • IKK-A
  • IKK-alpha
  • IkBKA
  • IkappaB kinase
  • CHUK
Background InformationInhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK), also known as, IKK-alpha (I-Kappa-B kinase-alpha), is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. CHUK plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. Mutation in CHUK has been associated with Cocoon syndrome.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-CHUK, clone 3G12H9 Antibody is an antibody against CHUK for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected CHUK in NIH/3T3 cells.
Biological Information
ImmunogenPurified recombinant fragment of human CHUK expressed in E. Coli.
Clone3G12H9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CHUK
  • IKKA
  • TCF16
Purification MethodUnpurified
UniProt Number
Molecular Weight~75 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji, Jurkat, THP-1 and K562 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected CHUK in Raji, Jurkat, THP-1 and K562 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE628 04055977171723