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ABE2620 Anti-CEP152 Antibody, C-Terminal

Anti-CEP152, C-Terminal, Cat. No. ABE2620, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152 kDa and has been tested in Immunofluorescence and Western Blotting.

Anti-CEP152 Antibody, C-Terminal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CEP152, KIAA90912 UniProt Number: O94986
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ABE2620
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2620
DescriptionAnti-CEP152 Antibody, C-Terminal
Alternate Names
  • Centrosomal protein of 152 kDa
Background InformationCentrosomal protein of 152 kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds CEP152 and CEP192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to centrosomes. This is a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S phase and elongate throughout S and G2 phase. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of CEP135, CEP295 (Ana1 analog) and Cep152 onto daughter centrioles.CEP152 is also an important regulator of genomic integrity and cellular response to DNA damage. Overexpression of CEP152 can drive amplification of centrioles. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical 'bird-head' facial appearance. CEP152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a result of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CEP152, C-Terminal, Cat. No. ABE2620, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 152 kDa and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected CEP152 in U2OS cells showing centrosome staining (Sonnen, K.F., et. al. (2013). J Cell Sci. 126(Pt 14):3223-33; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

Western Blotting Analysis: A representative lot detected CEP152 in cell lysates from transfected HEK293 cells (Sonnen, K.F., et. al. (2013). J Cell Sci. 126(Pt 14):3223-33).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 277 amino acids from the C-terminal half of human CEP152.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects the long isoform of human CEP152. It targets an epitope within 277 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CEP152
  • KIAA90912
Purification MethodProtein A purified
UniProt Number
Molecular Weight~ 230 kDa observed; 195.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CEP152 in 10 µg of U2OS cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2620 04054839193644