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217605 Anti-CD54 Mouse mAb (15.2)

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Anti-CD54 Mouse mAb (15.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ICAM-1, Domain 1

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217605
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217605-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
Catalogue Number217605
Brand Family Calbiochem®
SynonymsAnti-ICAM-1, Domain 1
References
ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.
Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Positive controlRaji cells
Preservative0.5 mg/ml gentamicin sulfate
Quality LevelMQ100
Applications
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Neutralization Studies
Application NotesFlow Cytometry (1-10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman monocytes
ImmunogenHuman
Clone15.2
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
217605-100UG 04055977202892

Documentation

Anti-CD54 Mouse mAb (15.2) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD54 Mouse mAb (15.2) Certificates of Analysis

TitleLot Number
217605

References

Reference overview
Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.
Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-ICAM-1, Domain 1
ApplicationFlow Cytometry (1-10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
BackgroundHuman CD54 (Intercellular Adhesion Molecule-1, or ICAM-1) is a ~90 kDa lymphocyte/monocyte cell surface glycoprotein that mediates cell-cell adhesion by interacting with the integrins LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18).
HostMouse
Immunogen speciesHuman
Immunogenhuman monocytes
Clone15.2
IsotypeIgG₁
Specieshuman
Positive controlRaji cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Purity≥95% by SDS-PAGE
Preservative0.5 mg/ml gentamicin sulfate
CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.
Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.