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MABF2053-100UG Anti-CD117 (c-Kit) Antibody, clone 2C11

This mouse monoclonal Anti-CD117 (c-Kit), clone 2C11 Antibody, Cat. No. MABF2053 is tested for use in Western Blotting and Flow Cytometry, for the detection of C-kit.

Anti-CD117 (c-Kit) Antibody, clone 2C11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: KIT, SCFR UniProt Number: P10721
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MABF2053-100UG
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2053-100UG
DescriptionAnti-CD117 (c-Kit) Antibody, clone 2C11
Alternate Names
  • Mast/stem cell growth factor receptor Kit
  • EC: 2.7.10.1
  • SCFR
  • Piebald trait protein
  • PBT
  • Proto-oncogene c-Kit
  • Tyrosine-protein kinase Kit
  • p145 c-kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Background InformationMast/stem cell growth factor receptor Kit (UniProt: P10721; also known as EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) is encoded by the KIT (also known as SCFR) gene (Gene ID: 3815) in human. C-Kit is a single-pass type I membrane protein with tyrosine protein kinase activity and serves as a cell-surface receptor for the cytokine KITLG/SCF and is shown to play a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis and other functions. c-Kit exists as a monomer in the absence of bound KITLG/SCF, however, it homodimerizes in the presence of bound KITLG/SCF and forms a heterotetramer with two KITLG/SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and KITLG/SCF binding enhances its autophosphorylation. Its kinase activity is down-regulated by phosphorylation on serine residues by PKC family members. Phosphorylation at Tyrosine 568 is essential for its interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to its internalization and degradation. c-Kit is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form with an extracellular domain (aa 26-524), a short transmembrane domain (aa 525-545), and a cytoplasmic domain (aa 546-976. Its two ATP-binding sites are localized to amino acids 596-603 and 671-677. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis mouse monoclonal Anti-CD117 (c-Kit), clone 2C11 Antibody, Cat. No. MABF2053 is tested for use in Western Blotting and Flow Cytometry, for the detection of C-kit.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected CD117 (c-Kit) in P815 cells (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).

Western Blotting Analysis: A representative lot detected CD117 (c-Kit) in DLD-1 cell lysate (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of Human C-kit.
Epitopeextracellular domain
Clone2C11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2C11 specifically detects CD117 (c-Kit) in human and murine cells. It targets an epitope with in the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KIT
  • SCFR
Purification MethodProtein G purified
UniProt Number
Molecular Weight~160 kDa observed; 109.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in DLD-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CD117 (c-Kit) in DLD-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2053-100UG 04054839529535